miR-150 regulates B lymphocyte in autoimmune hemolytic anemia/Evans syndrome by c-Myb

• Published in: International journal of hematology 2018-06, Vol.107 (6), p.666-672
• Main Authors: Xing, Limin, Xu, Wenyan, Qu, Yingying, Zhao, Manjun, Zhu, Hongli, Liu, Hong, Wang, Huaquan, Su, Xin, Shao, Zonghong
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Aged; Anemia; Anemia, Hemolytic, Autoimmune - blood; Anemia, Hemolytic, Autoimmune - immunology; Autoimmune hemolytic anemia; B-Lymphocytes - immunology; Bilirubin; Bilirubin - blood; c-Myb protein; Chronic lymphocytic leukemia; Complement C3 - metabolism; Complement component C3; Correlation analysis; Female; Gene Expression; Hematology; Hemoglobin; Hemoglobins; Hemolysis; Hemolytic anemia; Humans; Lymphocytes; Lymphocytes B; Male; Medicine; Medicine & Public Health; MicroRNAs - genetics; MicroRNAs - metabolism; MicroRNAs - physiology; Middle Aged; Oncology; Original Article; Patients; Polymerase chain reaction; Proto-Oncogene Proteins c-myb - genetics; Proto-Oncogene Proteins c-myb - metabolism; Real-Time Polymerase Chain Reaction; Remission; RNA, Messenger - genetics; RNA, Messenger - metabolism; Thrombocytopenia - immunology; Young Adult
• ISSN: 0925-5710; 1865-3774
• DOI: 10.1007/s12185-018-2429-z

The objective of the study was to study the regulation of B lymphocytes in patients with autoimmune hemolytic anemia (AIHA)/Evans syndrome. From October 2015 to May 2016, 35 patients with AIHA/Evans in the Department of Hematology, Tianjin Medical University General Hospital were enrolled into this study. c-Myb mRNA and miR-150 expression in B lymphocytes were measured using real-time PCR (RT-PCR). Correlation between c-Myb and miR-150 and clinical parameters in patients with AIHA/Evans were analyzed. c-Myb mRNA expression in hemolysis patients was significantly higher than in remission patients and CLL patients, negatively correlated with hemoglobin (Hb) level and complement 3(C3) levels, and positively correlated with total bilirubin (TBIL) concentration and indirect bilirubin (IBIL) concentration. miR-150 expression in hemolysis patients was significantly lower than in patients in remission and CLL patients. miR-150 was negatively correlated with TBIL and IBIL, and positively correlated with Hb, C3. c-Myb mRNA expression levels in hemolytic episode patients were negatively correlated with the expression levels of miR-150. The expression of c-Myb, a regulatory factor of B lymphocytes, is increased in B lymphocytes of AIHA/Evans patients, while miR-150 expression is decreased. c-Myb was negatively correlated with miR-150.