Morphological Control of Microtubule-Encapsulating Giant Vesicles by Changing Hydrostatic Pressure

For the development of artificial cell-like machinery, liposomes encapsulating cytoskeletons have drawn much recent attention. However, there has been no report showing isothermally reversible morphological changes of liposomes containing cytoskeletons. We succeeded in reversibly changing the shape...

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Bibliographic Details
Published in:Biological & pharmaceutical bulletin 2018/03/01, Vol.41(3), pp.288-293
Main Authors: Takiguchi, Kingo, Hayashi, Masahito, Kazayama, Yuki, Toyota, Taro, Harada, Yoshie, Nishiyama, Masayoshi
Format: Article
Language:English
Subjects:
Animals
artificial cell model
Artificial Cells
Biological membranes
Cell culture
Coated Vesicles
Cytoskeleton
Cytoskeleton - chemistry
Cytoskeleton - ultrastructure
Deep sea
Depolymerization
Drug Carriers
Drug Delivery Systems
Drug development
Encapsulation
External pressure
External stimuli
giant liposome
Hydrostatic Pressure
Lipid Bilayers
Lipids
Liposomes
Liposomes - chemistry
Membranes
microtubule
Microtubules
Microtubules - chemistry
Molecular modelling
Morphogenesis
Morphology
Osmotic Pressure
Osmotic stress
Particle Size
Polymerization
Pressure
Swine
Tubulin - chemistry
vesicle
Vesicles
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Summary:For the development of artificial cell-like machinery, liposomes encapsulating cytoskeletons have drawn much recent attention. However, there has been no report showing isothermally reversible morphological changes of liposomes containing cytoskeletons. We succeeded in reversibly changing the shape of cell-sized giant vesicles by controlling the polymerization/depolymerization state of cytoskeletal microtubules that were encapsulated in the vesicles using pressure changes. The result indicates that it is possible to manipulate artificial cell models composed of molecules such as lipids and proteins. The findings obtained in this study will be helpful in clarifying the details of cooperation between cytoskeletal dynamics and morphogenesis of biological membranes and in improving the design and construction of further advanced artificial cell-like machinery, such as drug-delivery systems. In addition, the experimental system used in this study can be applied to research to elucidate the adaptive strategy of living organisms to external stimuli and extreme conditions such as osmotic stress and high-pressure environments like the deep sea.
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.b17-00366