Detection of Distinct Changes in Gene-expression Profiles in Specimens of Tumors and Transition Zones of Tenascin-positive/-negative Head and Neck Squamous Cell Carcinoma

• Published in: Anticancer research 2018-03, Vol.38 (3), p.1279-1290
• Main Authors: Zivicova, Veronika, Gal, Peter, Mifkova, Alzbeta, Novak, Stepan, Kaltner, Herbert, Kolar, Michal, Strnad, Hynek, Sachova, Jana, Hradilova, Miluse, Chovanec, Martin, Gabius, Hans-Joachim, Smetana, JR, Karel, Fik, Zdenek
• Format: Article
• Language: English
• Subjects: Biomarkers, Tumor - genetics; Carcinoma, Squamous Cell - genetics; Carcinoma, Squamous Cell - metabolism; Carcinoma, Squamous Cell - surgery; Change detection; Disease-Free Survival; DNA microarrays; Fibronectin; Fibronectins - genetics; Fibronectins - metabolism; Galectin 1 - genetics; Galectin 1 - metabolism; Galectin-1; Gene expression; Gene Expression Profiling - methods; Gene Expression Regulation, Neoplastic; Gene Ontology; Genomes; Head; Head & neck cancer; Head and Neck Neoplasms - genetics; Head and Neck Neoplasms - metabolism; Head and Neck Neoplasms - surgery; Humans; Lipid metabolism; Lipids; Margins of Excision; Metabolism; Mucosa; Mucous Membrane - metabolism; Prognosis; Squamous cell carcinoma; Surgery; Tenascin; Tenascin - genetics; Tenascin - metabolism; Transcriptome; Tumors
• ISSN: 0250-7005; 1791-7530
• DOI: 10.21873/anticanres.12350

Having previously initiated genome-wide expression profiling in head and neck squamous cell carcinoma (HNSCC) for regions of the tumor, the margin of surgical resecate (MSR) and normal mucosa (NM), we here proceed with respective analysis of cases after stratification according to the expression status of tenascin (Ten). Tissue specimens of each anatomical site were analyzed by immunofluorescent detection of Ten, fibronectin (Fn) and galectin-1 (Gal-1) as well as by microarrays. Histopathological examination demonstrated that Ten Fn Gal-1 co-expression occurs more frequently in samples of HNSCC (55%) than in NM (9%; p