Type VII Collagen in the Human Accommodation System: Expression in Ciliary Body, Zonules, and Lens Capsule

To investigate intraocular expression of COL7A1 and its protein product type VII collagen, particularly at the accommodation system. Eyes from 26 human adult donors were used. COL7A1 expression was analyzed in ex vivo ciliary epithelium by microarray. Type VII collagen distribution was examined by W...

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Bibliographic Details
Published in:Investigative ophthalmology & visual science 2018-02, Vol.59 (2), p.1075-1083
Main Authors: Wullink, Bart, Pas, Hendri H, Van der Worp, Roelofje J, Schol, Martin, Janssen, Sarah F, Kuijer, Roel, Los, Leonoor I
Format: Article
Language:English
Subjects:
Accommodation, Ocular - physiology
Adult
Aged
Aged, 80 and over
Blotting, Western
Ciliary Body - metabolism
Ciliary Body - ultrastructure
Collagen Type VII - genetics
Collagen Type VII - metabolism
Female
Humans
Immunohistochemistry
Lens Capsule, Crystalline - metabolism
Lens Capsule, Crystalline - ultrastructure
Ligaments - metabolism
Ligaments - ultrastructure
Male
Microscopy, Immunoelectron
Middle Aged
Tissue Donors
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Summary:To investigate intraocular expression of COL7A1 and its protein product type VII collagen, particularly at the accommodation system. Eyes from 26 human adult donors were used. COL7A1 expression was analyzed in ex vivo ciliary epithelium by microarray. Type VII collagen distribution was examined by Western blot analysis, immunohistochemistry. and immuno-electron microscopy. COL7A1 is expressed by pigmented and nonpigmented ciliary epithelia. Type VII collagen is distributed particularly at the strained parts of the accommodation system. Type VII collagen was associated with various basement membranes and with ciliary zonules. Anchoring fibrils were not visualized. Type VII collagen distribution at strained areas suggests a supporting role in tissue integrity.
ISSN:1552-5783
1552-5783
DOI:10.1167/iovs.17-23425