Functionalized glass substrate for microarray analysis

• Published in: Thin solid films 2007-02, Vol.515 (7), p.4024-4031
• Main Authors: DeRosa, Rebecca L., Cardinale, Jean A., Cooper, Ashleigh
• Format: Article
• Language: English
• Subjects: Antibody adsorption; Condensed matter: structure, mechanical and thermal properties; Electron, ion, and scanning probe microscopy; Enzyme-linked immunosorbent assays (ELISA); Exact sciences and technology; General equipment and techniques; Instruments, apparatus, components and techniques common to several branches of physics and astronomy; Microarray; Physics; Sensors (chemical, optical, electrical, movement, gas, etc.); remote sensing; Solid surfaces and solid-solid interfaces; Structure and morphology; thickness; Structure of solids and liquids; crystallography; Surfaces and interfaces; thin films and whiskers (structure and nonelectronic properties); Thin film structure and morphology; X-ray photoelectron spectroscopy (XPS)
• ISSN: 0040-6090; 1879-2731
• DOI: 10.1016/j.tsf.2006.10.060

A series of glass treatments were investigated to develop a miniaturized enzyme-linked immunosorbent assay microarray sensor with covalently immobilized antibody. Two glass compositions were photo-hydrolytically treated and functionalized with an amino- or mercapto-silane. Surface characterization was done using contact angle, X-ray photoelectron spectroscopy and atomic force microscopy data. Antibody binding efficiency was statistically compared between treatments and glass compositions. Photo-hydrolytic treatment increased surface roughness and hydrophobic behavior due to alkali leaching and molecular surface rearrangement. Antibody binding tests indicated amino functionalized surfaces coupled with photo-hydrolytic treatment adsorb more antibody than those with thiol functionalization and without photo-hydrolytic treatment.