Fate of estrone in laboratory-scale constructed wetlands

• Published in: Ecological engineering 2018-02, Vol.111, p.60-68
• Main Authors: Hakk, Heldur, Sikora, Lawrence, Casey, Francis X.M.
• Format: Article
• Language: English
• Subjects: administered dose; Animal wastes; Aquatic plants; Araceae; Artificial wetlands; Carbon 14; Cells; Colloids; Constructed wetland; constructed wetlands; Dilution; Dissolved organic matter; drug residues; Duckweed; environmental fate; estriol; Estrogen; Estrone; Farm ponds; Fate and transport; Floating plants; Floating structures; Freshwater plants; Gravel; Horizontal surface flow wetland; Hormones; Influents; Interactions; liquids; Livestock; Manures; Mass spectrometry; Mass spectroscopy; Metabolites; Organic matter; pig manure; Removal; Sediment; Sediments; steroid hormones; Steroids; Storm seepage; subsurface flow; Swine; Thin layer chromatography; Vegetation; Vertical subsurface flow wetland; Wastewater; Wetlands
• ISSN: 0925-8574; 1872-6992
• DOI: 10.1016/j.ecoleng.2017.11.005

[Display omitted] •Laboratory-scale, four-cell constructed wetlands were spiked with [14C]estrone.•Overall, 95% removal of estrone was achieved with constructed wetland.•Highest removal of estrone (∼42%) occurred by sorption to vegetation in first cell.•At 7 d, 17% and 15% estrone partitioned to sediment and liquid layer, respectively.•After first cell, estrone movement was governed by particle-bound transport. A horizontal, subsurface, laboratory-scale constructed wetland (CW) consisting of four cells in series was used to determine the attenuation of the steroid hormone estrone (E1) present in animal wastewater. Liquid swine manure diluted 1:80 with farm pond water and dosed with [14C]E1 flowed through the series of cells containing floating vegetation (duckweed, family Lemnaceae) and a sand:gravel sediment layer. The aqueous layer within each cell was sampled across time, and at 168h duckweed and sediment were sampled for E1 and/or its metabolites. Control and Blank systems consisted of single cells with no vegetation or no vegetation/sediment, respectively. Only 5% of the dose was detected in the effluent at 168h. With the use of mass spectrometry and thin layer chromatography, it was determined that E1 was metabolized into estriol (E3) and to polar metabolites. The largest compartment for [14C] was duckweed (42.1% of administered dose) at 168h followed by sediment (17.3%) and the liquid layer (15.1% of the dose). Most of the vegetative removal of E1 occurred in the first cell, and thereafter, sediment:liquid layer interactions governed E1 movement suggesting particle-bound transport on either colloids or dissolved organic matter. Horizontal, subsurface flow CW were able to remove approximately 95% of the influent E1, and demonstrated the importance of vegetative matter in removal of this potent steroid hormone.