Micropterus salmoides rhabdovirus (MSRV) infection induced apoptosis and activated interferon signaling pathway in largemouth bass skin cells

• Published in: Fish & shellfish immunology 2018-05, Vol.76, p.161-166
• Main Authors: Gao, E-Bin, Chen, Guifang
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis; Bass; Cell Culture Techniques; Fish Diseases - immunology; Fish Diseases - virology; High-Throughput Nucleotide Sequencing - veterinary; Immunity, Innate - genetics; Interferon; Interferons - metabolism; Largemouth bass; Rhabdoviridae - physiology; Rhabdoviridae Infections - immunology; Rhabdoviridae Infections - veterinary; Rhabdoviridae Infections - virology; Rhabdovirus; Sequence Analysis, RNA - veterinary; Signal Transduction; Skin - immunology; Transcriptome - immunology; Transcriptomic profile
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2018.03.008

Largemouth bass (Micropterus salmoides) rhabdovirus (MSRV) was isolated from infected juveniles of largemouth bass, and the infected fish exhibited corkscrew, irregular swimming, and crooked body. To our knowledge, the potential molecular mechanisms underlying the pathogenesis of MSRV infection remain largely unknown. In the current study, we found that MSRV infection in largemouth bass skin (LBS) cells induced typical apoptosis, evidenced by the presence of apoptotic bodies and caspase-3 activation. To further analyze the host factors involved in MSRV infection in LBS cells, the transcriptomic profiles during MSRV infection were uncovered using deep RNA sequencing technique, and several differentially expressed genes (DEGs) were validated by quantitative PCR. Our results showed that a total of 124483 unigenes were assembled. Among them, 34465 and 27273 had significant hits to those in the NR and SwissProt databases. After MSRV infection, a total of 2432 and 2480 genes which involved in multiples pathways including TNF signaling, NF-κB signaling, Toll-like receptor signaling and RIG-I signaling pathway were differentially expressed in MSRV infected LBS cells compared to mock-infected cells at 12 h, respectively. Furthermore, quantitative PCR showed that the expression levels of 9 differentially expressed genes (DEGs) related to apoptosis and interferon signaling pathway was consistent with that from transcriptomic profiles. Together, our results not only demonstrated that interferon signaling pathway and apoptosis pathway might exerted crucial roles during MSRV infection, but also provided a useful resource for subsequent investigation of other immune-related genes related to virus infection. •MSRV infection induced typical apoptosis in LBS cells.•Multiple signaling pathways were involved in MSRV infection.•Apoptosis and interferon signaling related genes were differentially regulated by MSRV infection.