First identification of Cryptosporidium parvum subtype IIaA20G1R1 in water buffalos (Bubalus bubalis)

Cryptosporidium can infect a wide variety of vertebrate animals, including mammals, birds, amphibians, reptiles, and fish. There are few molecular characterizations of Cryptosporidium isolated from water buffalo. Thus, the present study investigated the occurrence and molecular characterization of C...

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Bibliographic Details
Published in:Research in veterinary science 2018-06, Vol.118, p.181-183
Main Authors: Martins, Thais Agostinho, Seixas, Mércia, Brito, Danilo Rodrigues Barros, Martins, Felippe Danyel Cardoso, Cardim, Sérgio Tosi, Melo, Priscilla, da Paixão Guterres, Sonália Ferreira, Patrício, Edjanio Gaspar, Garcia, João Luis
Format: Article
Language:English
Subjects:
Amphibians
Animals
Birds
Bubalus
Bubalus bubalis
Buffaloes
Calves
Cattle
Cattle Diseases - epidemiology
Cryptosporidiosis
Cryptosporidiosis - epidemiology
Cryptosporidium
Cryptosporidium parvum
Cryptosporidium parvum - classification
Cryptosporidium parvum - isolation & purification
Cryptosporidium ryanae
Deoxyribonucleic acid
DNA
DNA polymerase
Epidemiology
Feces - parasitology
Gene sequencing
Molecular chains
Molecular diagnosis
nested-PCR
Parasitic diseases
Reptiles
rRNA 18S
Studies
Veterinary medicine
Zoonoses
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Summary:Cryptosporidium can infect a wide variety of vertebrate animals, including mammals, birds, amphibians, reptiles, and fish. There are few molecular characterizations of Cryptosporidium isolated from water buffalo. Thus, the present study investigated the occurrence and molecular characterization of Cryptosporidium spp. in water buffalos by nested-PCR. Non-diarrheic feces were obtained from 122 water buffalo calves. All samples were tested by nested-PCR based on the 18S rRNA gene, after which positive samples were analyzed by RFLP and genetic sequencing. Sixteen fecal (13.1%) samples were positive, and RFLP showed that fifteen presented patterns consistent with C. ryanae and one with C. parvum. Sequencing of the gp60 gene from the C. parvum positive sample indicated the subtype IIaA20G1R1. This is the first identification of the IIaA20G1R1 subtype in water buffalos. •The present study, showed for the first time C. parvum subtype IIaA20G1R1 in water buffalo calves.•The most frequent Cryptosporidium species observed here was C. ryanae.•Cryptosporidium was detected in asymptomatic calves
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2018.02.002