Effect of dilution rate on productivity of continuous bacteriophage production in cellstat

Ability to efficiently propagate high quantities of bacteriophages (phages) is of great importance considering higher phage production needs in the future. Continuous production of phages could represent an interesting option. In our study, we tried to elucidate the effect of dilution rate on produc...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2018-04, Vol.102 (8), p.3649-3661
Main Authors: Nabergoj, Dominik, Kuzmić, Nina, Drakslar, Benjamin, Podgornik, Aleš
Format: Article
Language:English
Subjects:
Bacterial physiology
Bacteriophages
Bacteriophages - physiology
Biomedical and Life Sciences
Bioreactors
Biotechnological Products and Process Engineering
Biotechnology
Burst size
Chemical properties
Continuous production
Dilution
E coli
Escherichia coli K12 - virology
Latent period
Life Sciences
Mathematical models
Microbial Genetics and Genomics
Microbiology
Models, Biological
Optimization
Parameter sensitivity
Phages
Physiological aspects
Physiology
Productivity
Sensitivity analysis
Virus Cultivation - methods
Yeast
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Summary:Ability to efficiently propagate high quantities of bacteriophages (phages) is of great importance considering higher phage production needs in the future. Continuous production of phages could represent an interesting option. In our study, we tried to elucidate the effect of dilution rate on productivity of continuous production of phages in cellstat. As a model system, a well-studied phage T4 and Escherichia coli K-12 as a host were used. Experiments where physiology of bacteria was changing with dilution rate of cellstat and where bacterial physiology was kept constant were performed. For both setups there exists an optimal dilution rate when maximal productivity is achieved. Experimentally obtained values of phage concentration and corresponding productivity were compared with mathematical model predictions, and good agreement was obtained for both types of experiments. Analysis of mathematical model coefficients revealed that latent period and burst size to dilution rate coefficient mostly affect optimum dilution rate and productivity. Due to high sensitivity, it is important to evaluate phage growth parameters carefully, to run cellstat under optimal productivity.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-018-8893-9