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Bioremediation of coastal areas 5 years after the Nakhodka oil spill in the Sea of Japan: isolation and characterization of hydrocarbon-degrading bacteria
Five years after the 1997 Nakhodka oil spill in the Sea of Japan, seven bacterial strains capable of utilizing the heavy oil spilled from the Nakhodka Russian oil tanker were isolated from three coastal areas (namely Katano Seashore of Fukui Prefecture, Osawa and Atake seashores of Ishikawa Prefectu...
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Published in: | Environment international 2004-09, Vol.30 (7), p.911-922 |
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description | Five years after the 1997
Nakhodka oil spill in the Sea of Japan, seven bacterial strains capable of utilizing the heavy oil spilled from the
Nakhodka Russian oil tanker were isolated from three coastal areas (namely Katano Seashore of Fukui Prefecture, Osawa and Atake seashores of Ishikawa Prefecture) and the
Nakhodka Russian oil tanker after a 5-year bioremediation process. All bacterial strains isolated could utilize long-chain-length alkanes efficiently, but not aromatic, and all of them were able to grow well on heavy oil. Using 16S rDNA sequencing, most of the strains were affiliated to
Pseudomonas aeruginosa. Comparing between the year 1997 (at the beginning of bioremediation process) and the year 2001 (after 5 years of bioremediation), there was no significant change in morphology and size of hydrocarbon-degrading bacteria during the 5-year bioremediation. Scanning and transmission electron microscopic observations revealed that a large number of hydrocarbon-degrading bacteria still existed in the sites consisting of a variety of morphological forms of bacteria, such as coccus (
Streptococcus and
Staphylococcus) and bacillus (
Streptobacillus). On the application of bioremediation processes on the laboratory-scale, laboratory microcosm experiments (containing seawater, beach sand, and heavy oil) under aerobic condition by two different treatments (i.e., placed the inside building and the outside building) were established for bioremediation of heavy oil to investigate the significance of the role of hydrocarbon-degrading bacteria on them. There was no significant bacterial activity differentiation in the two treatments, and removal of heavy oil by hydrocarbon-degrading bacteria in the outside building was slightly greater than that in the inside building. The values of pH, Eh, EC, and dissolved oxygen (DO) in two treatments indicated that the bioremediation process took place under aerobic conditions (DO: 1–6 mg/l; Eh: 12–300 mV) and neutral-alkaline conditions (pH 6.4–8) with NaCl concentrations of 3–15% (ECs of 45–200 mS/cm). |
doi_str_mv | 10.1016/j.envint.2004.02.007 |
format | article |
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Nakhodka oil spill in the Sea of Japan, seven bacterial strains capable of utilizing the heavy oil spilled from the
Nakhodka Russian oil tanker were isolated from three coastal areas (namely Katano Seashore of Fukui Prefecture, Osawa and Atake seashores of Ishikawa Prefecture) and the
Nakhodka Russian oil tanker after a 5-year bioremediation process. All bacterial strains isolated could utilize long-chain-length alkanes efficiently, but not aromatic, and all of them were able to grow well on heavy oil. Using 16S rDNA sequencing, most of the strains were affiliated to
Pseudomonas aeruginosa. Comparing between the year 1997 (at the beginning of bioremediation process) and the year 2001 (after 5 years of bioremediation), there was no significant change in morphology and size of hydrocarbon-degrading bacteria during the 5-year bioremediation. Scanning and transmission electron microscopic observations revealed that a large number of hydrocarbon-degrading bacteria still existed in the sites consisting of a variety of morphological forms of bacteria, such as coccus (
Streptococcus and
Staphylococcus) and bacillus (
Streptobacillus). On the application of bioremediation processes on the laboratory-scale, laboratory microcosm experiments (containing seawater, beach sand, and heavy oil) under aerobic condition by two different treatments (i.e., placed the inside building and the outside building) were established for bioremediation of heavy oil to investigate the significance of the role of hydrocarbon-degrading bacteria on them. There was no significant bacterial activity differentiation in the two treatments, and removal of heavy oil by hydrocarbon-degrading bacteria in the outside building was slightly greater than that in the inside building. The values of pH, Eh, EC, and dissolved oxygen (DO) in two treatments indicated that the bioremediation process took place under aerobic conditions (DO: 1–6 mg/l; Eh: 12–300 mV) and neutral-alkaline conditions (pH 6.4–8) with NaCl concentrations of 3–15% (ECs of 45–200 mS/cm).</description><identifier>ISSN: 0160-4120</identifier><identifier>EISSN: 1873-6750</identifier><identifier>DOI: 10.1016/j.envint.2004.02.007</identifier><identifier>PMID: 15196839</identifier><identifier>CODEN: ENVIDV</identifier><language>eng</language><publisher>Amsterdam: Elsevier Ltd</publisher><subject>Applied sciences ; Bacillus ; Bacteria - genetics ; Bacteria - metabolism ; Bacteria - ultrastructure ; Biodegradation of pollutants ; Biodegradation, Environmental ; Biological and medical sciences ; Bioremediation ; Biotechnology ; Coccus ; Disasters ; Earth sciences ; Earth, ocean, space ; Engineering and environment geology. Geothermics ; Environment and pollution ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; Heavy oil ; Hydrocarbon-degrading bacteria ; Industrial applications and implications. Economical aspects ; Japan ; Microscopy, Electron ; Nakhodka Russian oil tanker ; Natural water pollution ; Oceans and Seas ; Petroleum - metabolism ; Pollution ; Pollution, environment geology ; Pseudomonas aeruginosa ; RNA, Ribosomal, 16S - genetics ; Seawater - analysis ; Seawaters, estuaries ; Sequence Analysis, DNA ; Ships ; Spectrometry, X-Ray Emission ; Staphylococcus ; Streptobacillus ; Streptococcus ; Water Microbiology ; Water Pollutants, Chemical - metabolism ; Water Pollution, Chemical - prevention & control ; Water treatment and pollution</subject><ispartof>Environment international, 2004-09, Vol.30 (7), p.911-922</ispartof><rights>2004 Elsevier Ltd</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a539t-836402e5266a8479761842ed456d48c6f4182abd568a84ad9eac72487181cc553</citedby><cites>FETCH-LOGICAL-a539t-836402e5266a8479761842ed456d48c6f4182abd568a84ad9eac72487181cc553</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15858070$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15196839$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chaerun, S.Khodijah</creatorcontrib><creatorcontrib>Tazaki, Kazue</creatorcontrib><creatorcontrib>Asada, Ryuji</creatorcontrib><creatorcontrib>Kogure, Kazuhiro</creatorcontrib><title>Bioremediation of coastal areas 5 years after the Nakhodka oil spill in the Sea of Japan: isolation and characterization of hydrocarbon-degrading bacteria</title><title>Environment international</title><addtitle>Environ Int</addtitle><description>Five years after the 1997
Nakhodka oil spill in the Sea of Japan, seven bacterial strains capable of utilizing the heavy oil spilled from the
Nakhodka Russian oil tanker were isolated from three coastal areas (namely Katano Seashore of Fukui Prefecture, Osawa and Atake seashores of Ishikawa Prefecture) and the
Nakhodka Russian oil tanker after a 5-year bioremediation process. All bacterial strains isolated could utilize long-chain-length alkanes efficiently, but not aromatic, and all of them were able to grow well on heavy oil. Using 16S rDNA sequencing, most of the strains were affiliated to
Pseudomonas aeruginosa. Comparing between the year 1997 (at the beginning of bioremediation process) and the year 2001 (after 5 years of bioremediation), there was no significant change in morphology and size of hydrocarbon-degrading bacteria during the 5-year bioremediation. Scanning and transmission electron microscopic observations revealed that a large number of hydrocarbon-degrading bacteria still existed in the sites consisting of a variety of morphological forms of bacteria, such as coccus (
Streptococcus and
Staphylococcus) and bacillus (
Streptobacillus). On the application of bioremediation processes on the laboratory-scale, laboratory microcosm experiments (containing seawater, beach sand, and heavy oil) under aerobic condition by two different treatments (i.e., placed the inside building and the outside building) were established for bioremediation of heavy oil to investigate the significance of the role of hydrocarbon-degrading bacteria on them. There was no significant bacterial activity differentiation in the two treatments, and removal of heavy oil by hydrocarbon-degrading bacteria in the outside building was slightly greater than that in the inside building. The values of pH, Eh, EC, and dissolved oxygen (DO) in two treatments indicated that the bioremediation process took place under aerobic conditions (DO: 1–6 mg/l; Eh: 12–300 mV) and neutral-alkaline conditions (pH 6.4–8) with NaCl concentrations of 3–15% (ECs of 45–200 mS/cm).</description><subject>Applied sciences</subject><subject>Bacillus</subject><subject>Bacteria - genetics</subject><subject>Bacteria - metabolism</subject><subject>Bacteria - ultrastructure</subject><subject>Biodegradation of pollutants</subject><subject>Biodegradation, Environmental</subject><subject>Biological and medical sciences</subject><subject>Bioremediation</subject><subject>Biotechnology</subject><subject>Coccus</subject><subject>Disasters</subject><subject>Earth sciences</subject><subject>Earth, ocean, space</subject><subject>Engineering and environment geology. Geothermics</subject><subject>Environment and pollution</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Heavy oil</subject><subject>Hydrocarbon-degrading bacteria</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Japan</subject><subject>Microscopy, Electron</subject><subject>Nakhodka Russian oil tanker</subject><subject>Natural water pollution</subject><subject>Oceans and Seas</subject><subject>Petroleum - metabolism</subject><subject>Pollution</subject><subject>Pollution, environment geology</subject><subject>Pseudomonas aeruginosa</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Seawater - analysis</subject><subject>Seawaters, estuaries</subject><subject>Sequence Analysis, DNA</subject><subject>Ships</subject><subject>Spectrometry, X-Ray Emission</subject><subject>Staphylococcus</subject><subject>Streptobacillus</subject><subject>Streptococcus</subject><subject>Water Microbiology</subject><subject>Water Pollutants, Chemical - metabolism</subject><subject>Water Pollution, Chemical - prevention & control</subject><subject>Water treatment and pollution</subject><issn>0160-4120</issn><issn>1873-6750</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNqFkcuOEzEQRVsIxISBP0DIG9h1Y7v9CgskGPHUCBbA2qrY1RNnOnbG7owUPoWvxaEjYAWrWtSpW6U6TfOY0Y5Rpp5vOoy3IU4dp1R0lHeU6jvNghndt0pLerdZVIy2gnF61jwoZUMp5cLI-80Zk2ypTL9cND9eh5Rxiz7AFFIkaSAuQZlgJJARCpHkgJALgWHCTKY1kk9wvU7-GkgKIym7MI4kxF-dLwjHgI-wg_iChJLGORSiJ24NGVzNCN9_b1offE4O8irF1uNVBh_iFVnNGDxs7g0wFnx0qufNt7dvvl68by8_v_tw8eqyBdkvp9b0SlCOkisFRuilVswIjl5I5YVxahDMcFh5qUztg18iOF3_oJlhzknZnzfP5txdTjd7LJPdhuJwHCFi2hfLKeOiV-q_IBOaMalFBcUMupxKyTjYXQ5byAfLqD3Ksxs7y7NHeZZyW-XVsSen_P2qGvkzdLJVgacnAIqDccgQXSh_cUYaqmnlXs4c1rfdBsy2uIDRVcsZ3WR9Cv--5Cfir7rp</recordid><startdate>20040901</startdate><enddate>20040901</enddate><creator>Chaerun, S.Khodijah</creator><creator>Tazaki, Kazue</creator><creator>Asada, Ryuji</creator><creator>Kogure, Kazuhiro</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>C1K</scope><scope>SOI</scope><scope>7QH</scope><scope>7QO</scope><scope>7T7</scope><scope>7TV</scope><scope>7UA</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H97</scope><scope>L.G</scope><scope>P64</scope></search><sort><creationdate>20040901</creationdate><title>Bioremediation of coastal areas 5 years after the Nakhodka oil spill in the Sea of Japan: isolation and characterization of hydrocarbon-degrading bacteria</title><author>Chaerun, S.Khodijah ; Tazaki, Kazue ; Asada, Ryuji ; Kogure, Kazuhiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a539t-836402e5266a8479761842ed456d48c6f4182abd568a84ad9eac72487181cc553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Applied sciences</topic><topic>Bacillus</topic><topic>Bacteria - genetics</topic><topic>Bacteria - metabolism</topic><topic>Bacteria - ultrastructure</topic><topic>Biodegradation of pollutants</topic><topic>Biodegradation, Environmental</topic><topic>Biological and medical sciences</topic><topic>Bioremediation</topic><topic>Biotechnology</topic><topic>Coccus</topic><topic>Disasters</topic><topic>Earth sciences</topic><topic>Earth, ocean, space</topic><topic>Engineering and environment geology. Geothermics</topic><topic>Environment and pollution</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Heavy oil</topic><topic>Hydrocarbon-degrading bacteria</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Japan</topic><topic>Microscopy, Electron</topic><topic>Nakhodka Russian oil tanker</topic><topic>Natural water pollution</topic><topic>Oceans and Seas</topic><topic>Petroleum - metabolism</topic><topic>Pollution</topic><topic>Pollution, environment geology</topic><topic>Pseudomonas aeruginosa</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Seawater - analysis</topic><topic>Seawaters, estuaries</topic><topic>Sequence Analysis, DNA</topic><topic>Ships</topic><topic>Spectrometry, X-Ray Emission</topic><topic>Staphylococcus</topic><topic>Streptobacillus</topic><topic>Streptococcus</topic><topic>Water Microbiology</topic><topic>Water Pollutants, Chemical - metabolism</topic><topic>Water Pollution, Chemical - prevention & control</topic><topic>Water treatment and pollution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chaerun, S.Khodijah</creatorcontrib><creatorcontrib>Tazaki, Kazue</creatorcontrib><creatorcontrib>Asada, Ryuji</creatorcontrib><creatorcontrib>Kogure, Kazuhiro</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><collection>Aqualine</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Pollution Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Environment international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chaerun, S.Khodijah</au><au>Tazaki, Kazue</au><au>Asada, Ryuji</au><au>Kogure, Kazuhiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bioremediation of coastal areas 5 years after the Nakhodka oil spill in the Sea of Japan: isolation and characterization of hydrocarbon-degrading bacteria</atitle><jtitle>Environment international</jtitle><addtitle>Environ Int</addtitle><date>2004-09-01</date><risdate>2004</risdate><volume>30</volume><issue>7</issue><spage>911</spage><epage>922</epage><pages>911-922</pages><issn>0160-4120</issn><eissn>1873-6750</eissn><coden>ENVIDV</coden><abstract>Five years after the 1997
Nakhodka oil spill in the Sea of Japan, seven bacterial strains capable of utilizing the heavy oil spilled from the
Nakhodka Russian oil tanker were isolated from three coastal areas (namely Katano Seashore of Fukui Prefecture, Osawa and Atake seashores of Ishikawa Prefecture) and the
Nakhodka Russian oil tanker after a 5-year bioremediation process. All bacterial strains isolated could utilize long-chain-length alkanes efficiently, but not aromatic, and all of them were able to grow well on heavy oil. Using 16S rDNA sequencing, most of the strains were affiliated to
Pseudomonas aeruginosa. Comparing between the year 1997 (at the beginning of bioremediation process) and the year 2001 (after 5 years of bioremediation), there was no significant change in morphology and size of hydrocarbon-degrading bacteria during the 5-year bioremediation. Scanning and transmission electron microscopic observations revealed that a large number of hydrocarbon-degrading bacteria still existed in the sites consisting of a variety of morphological forms of bacteria, such as coccus (
Streptococcus and
Staphylococcus) and bacillus (
Streptobacillus). On the application of bioremediation processes on the laboratory-scale, laboratory microcosm experiments (containing seawater, beach sand, and heavy oil) under aerobic condition by two different treatments (i.e., placed the inside building and the outside building) were established for bioremediation of heavy oil to investigate the significance of the role of hydrocarbon-degrading bacteria on them. There was no significant bacterial activity differentiation in the two treatments, and removal of heavy oil by hydrocarbon-degrading bacteria in the outside building was slightly greater than that in the inside building. The values of pH, Eh, EC, and dissolved oxygen (DO) in two treatments indicated that the bioremediation process took place under aerobic conditions (DO: 1–6 mg/l; Eh: 12–300 mV) and neutral-alkaline conditions (pH 6.4–8) with NaCl concentrations of 3–15% (ECs of 45–200 mS/cm).</abstract><cop>Amsterdam</cop><pub>Elsevier Ltd</pub><pmid>15196839</pmid><doi>10.1016/j.envint.2004.02.007</doi><tpages>12</tpages></addata></record> |
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subjects | Applied sciences Bacillus Bacteria - genetics Bacteria - metabolism Bacteria - ultrastructure Biodegradation of pollutants Biodegradation, Environmental Biological and medical sciences Bioremediation Biotechnology Coccus Disasters Earth sciences Earth, ocean, space Engineering and environment geology. Geothermics Environment and pollution Exact sciences and technology Fundamental and applied biological sciences. Psychology Heavy oil Hydrocarbon-degrading bacteria Industrial applications and implications. Economical aspects Japan Microscopy, Electron Nakhodka Russian oil tanker Natural water pollution Oceans and Seas Petroleum - metabolism Pollution Pollution, environment geology Pseudomonas aeruginosa RNA, Ribosomal, 16S - genetics Seawater - analysis Seawaters, estuaries Sequence Analysis, DNA Ships Spectrometry, X-Ray Emission Staphylococcus Streptobacillus Streptococcus Water Microbiology Water Pollutants, Chemical - metabolism Water Pollution, Chemical - prevention & control Water treatment and pollution |
title | Bioremediation of coastal areas 5 years after the Nakhodka oil spill in the Sea of Japan: isolation and characterization of hydrocarbon-degrading bacteria |
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