A novel acidophile community populating waste ore deposits at an acid mine drainage site

• Published in: Journal of environmental sciences (China) 2007, Vol.19 (4), p.444-450
• Main Authors: HAO, Chun-bo, ZHANG, Hong-xun, BAI, Zhi-hui, HU, Qing, ZHANG, Bao-guo
• Format: Article
• Language: English
• Subjects: 16S rRNA; acid mine drainage; acidophile; Actinobacteria; Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Base Sequence; DGGE; DNA, Bacterial - genetics; Environmental Microbiology; Freshwater; Industrial Waste; Mining; Molecular Sequence Data; Phylogeny; Planctomycetes; Proteobacteria; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; waste ore; 嗜酸群落; 废矿堆积; 微生物; 酸性矿山排水场址
• ISSN: 1001-0742; 1878-7320
• DOI: 10.1016/S1001-0742(07)60074-6

Waste ore samples （pH 3.0） were collected at an acid mine drainage （AMD） site in Anhui, China. The present acidophillc microbial community in the waste ore was studied with 16S rRNA gene clone library and denaturing gradient gel electrophoresis （DGGE）. Eighteen different clones were identified and affiliated withActinobacteria, low G ＋ C Gram-positives, Thermomicrobia, Acidobacteria, Proteobacteria, candidate division TM7, and Planctomycetes. Phylogenetic analysis of 16S rRNA gene sequences revealed a diversity of acidophiles in the samples that were mostly novel. It is unexpected that the moderately thermophilic acidophiles were abundant in the acidic ecosystem and may play a great role in the generation of AMD. The result of DGGE was consistent with that of clone library analysis. These findings help in the better understanding of the generation mechanism of AMD and in developing a more efficient method to control AMD.