An apple NAC transcription factor enhances salt stress tolerance by modulating the ethylene response

It is known that ethylene signaling is involved in the regulation of the salt stress response. However, the molecular mechanism of ethylene‐regulated salt stress tolerance remains largely unclear. In this study, an apple NAM ATAF CUC transcription factor, MdNAC047, was isolated and functionally char...

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Published in:Physiologia plantarum 2018-11, Vol.164 (3), p.279-289
Main Authors: An, Jian‐Ping, Yao, Ji‐Fang, Xu, Rui‐Rui, You, Chun‐Xiang, Wang, Xiao‐Fei, Hao, Yu‐Jin
Format: Article
Language:English
Subjects:
Abiotic stress
Assaying
Cellular stress response
Electrophoretic mobility
Electrophoretic Mobility Shift Assay
Ethylene
Ethylenes - metabolism
Gene expression
Gene Expression Regulation, Plant - genetics
Gene Expression Regulation, Plant - physiology
Genetic analysis
Malus - drug effects
Malus - genetics
Malus - metabolism
Plant Proteins - genetics
Plant Proteins - metabolism
Salinity tolerance
Salt
Salt Tolerance
Stress
Stress, Physiological - drug effects
Stresses
Transcription factors
Transcription Factors - genetics
Transcription Factors - metabolism
Yeast
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Summary:It is known that ethylene signaling is involved in the regulation of the salt stress response. However, the molecular mechanism of ethylene‐regulated salt stress tolerance remains largely unclear. In this study, an apple NAM ATAF CUC transcription factor, MdNAC047, was isolated and functionally characterized to be involved in ethylene‐modulated salt tolerance. MdNAC047 gene was significantly induced by salt treatment and its overexpression conferred increased tolerance to salt stress and facilitated the release of ethylene. Quantitative real‐time‐PCR analysis demonstrated that overexpression of MdNAC047 increased the expression of ethylene‐responsive genes. Electrophoretic mobility shift assay, yeast one‐hybrid and dual‐luciferase assays suggested that MdNAC047 directly binds to the MdERF3 (ETHYLENE RESPONSE FACTOR) promoter and activates its transcription. In addition, genetic analysis assays indicated that MdNAC047 regulates ethylene production at least partially in an MdERF3‐dependent pathway. Overall, we found a novel ‘MdNAC047‐MdERF3‐ethylene‐salt tolerance’ regulatory pathway, which provide new insight into the link between ethylene and salt stress.
ISSN:0031-9317
1399-3054
DOI:10.1111/ppl.12724