Effect of preameloblast-conditioned medium and CPNE7 on root surfaces in dogs: a histologic and histomorphometric evaluation

• Published in: Journal of molecular histology 2018-06, Vol.49 (3), p.265-276
• Main Authors: Yu, Sang-Joun, Lee, Yoon Seon, Choung, Han-Wool, Park, Yeoung-Hyun, Kim, Byung-Ock, Park, Joo-Cheol
• Format: Article
• Language: English
• Subjects: Ankylosis; Biomedicine; Cell Biology; Cementum; Dental research; Dental roots; Dentin; Dentistry; Developmental Biology; Endodontics; Estrogens; Growth factors; Histology; Life Sciences; Mandible; Original Paper; Periodontics; Stem cells; Teeth; Tissue engineering
• ISSN: 1567-2379; 1567-2387
• DOI: 10.1007/s10735-018-9766-3

Preameloblast-conditioned medium (PACM) has been reported as a potent dentin regenerative material, but its effects as a mixture on periodontal regeneration and the role of CPNE7 in PACM are not known. The purpose of this study is to evaluate the histologic and histomorphometric effects of preameloblast-conditioned medium (PACM) and CPNE7 on periodontal tissue healing in dogs. Seventy-two mandibular premolar roots from ten dogs were extracted and randomly divided into six groups (n = 12 each): (1) positive control group; (2) negative control group; (3) cementum-removed and PACM-treated group; (4) cementum-preserved and PACM-treated group; (5) CPNE7-inactivated PACM-treated group; and (6) recombinant CPNE7-treated group. The extracted roots were replanted into extraction sockets for 4 and 8 weeks and analyzed histologically. Most of the root surfaces in the negative control group showed ankylosis; and those in the experimental groups showed newly formed PDL-like and cementum-like tissues. Histomorphometric analysis of horizontal sections showed that the mean length of the PDL on the roots of the positive controls was similar to those in cementum-removed or -preserved and PACM-treated group at 8 weeks ( p  = 1.08). Sagittal sections showed that the mean length of the new cementum on the roots in cementum-removed and PACM-treated group was significantly greater than that in CPNE7-inactivated PACM-treated group ( p  = 0.037). The mean length of the newly formed PDL on the roots in CPNE7- inactivated PACM-treated and rCPNE7-treated groups was significantly greater than that in the negative controls at 8 weeks ( p  = 0.037, p  = 0.036). The use of PACM and CPNE7 in tooth replantation resulted in increased PDL and cementum formation, suggesting the beneficial role of PACM and CPNE7 in periodontal tissue healing.