On-chip Capillary Electrophoresis Fractionation of DNA Construct for Cell-free Protein Expression

An on-chip capillary electrophoresis-based DNA collection was applied to isolate desired DNA construct from a reaction mixture of DNA-assembly instead of traditional living cell-based screening. The isolated construct was amplified with polymerase chain reaction and subjected to a coupled cell-free...

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Bibliographic Details
Published in:Chemistry letters 2007-11, Vol.36 (11), p.1346-1347
Main Authors: Nojima, Takahiko, Kaneda, Shohei, Fujii, Teruo
Format: Article
Language:English
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Summary:An on-chip capillary electrophoresis-based DNA collection was applied to isolate desired DNA construct from a reaction mixture of DNA-assembly instead of traditional living cell-based screening. The isolated construct was amplified with polymerase chain reaction and subjected to a coupled cell-free transcription/translation system as a template, and the template-dependent protein expression was observed. It was demonstrated that total process of standard recombinant DNA technology was performed in a cell-free condition.
ISSN:0366-7022
1348-0715
DOI:10.1246/cl.2007.1346