Antimutagenic properties of a polyphenol-enriched extract derived from sesame-seed perisperm

A polyphenolic mixture derived from sesame-seed perisperm (SSP) strongly reduced the mutagenicity of hydrogen peroxide (H 2O 2), sodium azide (NaN 3), and benzo[a]pyrene (BaP) in strains TA100 and/or TA98 of Salmonella typhimurium. It exhibited desmutagenic activity against H 2O 2, BaP in TA98 and/o...

Full description

Saved in:
Bibliographic Details
Published in:Mutation research. Genetic toxicology and environmental mutagenesis 2007-12, Vol.634 (1), p.163-171
Main Authors: Lazarou, Despoina, Grougnet, Raphael, Papadopoulos, Athanasios
Format: Article
Language:English
Subjects:
Ames’ test
Antimutagenicity
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
Medical sciences
Polyphenol extract
Salmonella typhimurium
Sesame-seed perisperm
Toxicology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A polyphenolic mixture derived from sesame-seed perisperm (SSP) strongly reduced the mutagenicity of hydrogen peroxide (H 2O 2), sodium azide (NaN 3), and benzo[a]pyrene (BaP) in strains TA100 and/or TA98 of Salmonella typhimurium. It exhibited desmutagenic activity against H 2O 2, BaP in TA98 and/or TA100 and biomutagenic activity (apparently by affecting the DNA-repair system) against NaN 3 in strain TA100. According to in vitro experiments the polyphenolic mixture inhibited the activity of the CYP1A1 (EROD) enzyme responsible for the activation of BaP in the Ames’ test, as well as that of the cytosolic enzyme GST. A cytosolic fraction from liver of male Wistar rats treated with either 20% SSP in the food, or 3 mg or 6 mg of polyphenolic mixture/20 g food/day for a time period of 8 weeks reduced the mutagenic potential of BaP in strains TA100 and TA98, with the cytosolic fraction from rats treated with SSP causing the strongest reduction. Furthermore, a microsomal fraction from the 20% SSP-treated rats inhibited the mutagenicity of BaP in strains TA100 (26.3%) and TA98 (23%). In contrast, a microsomal fraction from rats treated with 3 mg of polyphenolic mixture stimulated the mutagenicity of BaP in TA100 but reduced it in TA98, while for the microsomal fraction from rats treated with 6 mg of polyphenolic mixture, these effects on TA100 and TA98 were reversed.
ISSN:1383-5718
1879-3592
DOI:10.1016/j.mrgentox.2007.07.008