Efficacy of poly(lactic acid)/carvacrol electrospun membranes against Staphylococcus aureus and Candida albicans in single and mixed cultures

• Published in: Applied microbiology and biotechnology 2018-05, Vol.102 (9), p.4171-4181
• Main Authors: Scaffaro, Roberto, Lopresti, Francesco, D’Arrigo, Manuela, Marino, Andreana, Nostro, Antonia
• Format: Article
• Language: English
• Subjects: Anti-Infective Agents - pharmacology; Antiinfectives and antibacterials; Antimicrobial activity; Antimicrobial agents; Applied Microbial and Cell Physiology; Biofilms; Biofilms - drug effects; Biological activity; Biomedical and Life Sciences; Biotechnology; Candida albicans; Candida albicans - drug effects; Carvacrol; Electrospinning; Essential oils; Feasibility studies; Life Sciences; Mechanical properties; Membranes; Membranes, Artificial; Microbial Genetics and Genomics; Microbial Sensitivity Tests; Microbiology; Monoterpenes - pharmacology; New technology; Polyesters - pharmacology; Polylactic acid; Skin; Staphylococcus aureus; Staphylococcus aureus - drug effects; Wounds
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-018-8879-7

Carvacrol (CAR) is one of the most promising essential oil components with antimicrobial activity. New technologies aimed to incorporate this active molecule into carrier matrix to improve the stability and prolong the biological activity. The goal of this study was to investigate the feasibility of incorporating CAR into electrospun membranes of poly(lactic acid) (PLA) for potential applications as active antimicrobial system. To this end, PLA membranes containing homogeneously dispersed CAR were successfully prepared and a series of systematic tests including morpho-mechanical properties, in vitro release rate, and antimicrobial/antibiofilm activities against Staphylococcus aureus and Candida albicans were carried out. The results revealed that CAR has a good compatibility with PLA and acts as a plasticizer, improving flexibility and extensibility of the matrix. The gradual release of CAR from PLA membranes warranted a significant antimicrobial activity up to 144 h and reduced the biofilm production by 92–96 and 88–95% of S. aureus and C. albicans in single and mixed cultures. A strong decrease of cell count, biomass, metabolic activity, and vitality of established 24- and 48-h biofilms were also demonstrated. In conclusion, this work highlights the potential of electrospun nanofibrous membranes as efficient stabilizers-carriers of CAR and opens up interesting perspectives on the use of this system as new tool for skin and wound bacterial–fungal infections.