Phosphate Transporter PvPht1;2 Enhances Phosphorus Accumulation and Plant Growth without Impacting Arsenic Uptake in Plants

Phosphorus is an important macronutrient for plant growth and is acquired by plants mainly as phosphate (P). Phosphate transporters (Phts) are responsible for P and arsenate (AsV) uptake in plants including arsenic-hyperaccumulator Pteris vittata. P. vittata is efficient in AsV uptake and P utilizat...

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Bibliographic Details
Published in:Environmental science & technology 2018-04, Vol.52 (7), p.3975-3981
Main Authors: Cao, Yue, Sun, Dan, Chen, Jun-Xiu, Mei, Hanyi, Ai, Hao, Xu, Guohua, Chen, Yanshan, Ma, Lena Q
Format: Article
Language:English
Subjects:
Accumulation
Agricultural production
Arsenates
Arsenic
Crop production
Crops
Food production
Food safety
Molecular chains
Molecules
Phosphate transporter
Phosphates
Phosphorus
Plant growth
Soil permeability
Tobacco
Transgenic plants
Translocation
Xylem
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Summary:Phosphorus is an important macronutrient for plant growth and is acquired by plants mainly as phosphate (P). Phosphate transporters (Phts) are responsible for P and arsenate (AsV) uptake in plants including arsenic-hyperaccumulator Pteris vittata. P. vittata is efficient in AsV uptake and P utilization, but the molecular mechanism of its P uptake is largely unknown. In this study, a P. vittata Pht, PvPht1;2 , was cloned and transformed into tobacco (Nicotiana tabacum). In hydroponic experiments, all transgenic lines displayed markedly higher P content and better growth than wild type, suggesting that PvPht1;2 mediated P uptake in plants. In addition, expressing PvPht1;2 also increased the shoot/root 32P ratio by 69–92% and enhanced xylem sap P by 46–62%, indicating that PvPht1;2 also mediated P translocation in plants. Unlike many Phts permeable to AsV, PvPht1;2 showed little ability to transport AsV. In soil experiments, PvPht1;2 also significantly increased shoot biomass without elevating As accumulation in PvPht1;2 transgenic tobacco. Taken together, our results demonstrated that PvPht1;2 is a specific P transporter responsible for P acquisition and translocation in plants. We envisioned that PvPht1;2 can enhance crop P acquisition without impacting AsV uptake, thereby increasing crop production without compromising food safety.
ISSN:0013-936X
1520-5851
DOI:10.1021/acs.est.7b06674