Effects of Selective Cyclooxygenase‐2 Inhibitor and Omega‐3 Fatty Acid on Serum Interleukin‐1β, Osteocalcin, and C‐Reactive Protein Levels in Rats

• Published in: Journal of periodontology (1970) 2006-04, Vol.77 (4), p.657-663
• Main Authors: Vardar‐Şengül, Saynur, Buduneli, Nurcan, Buduneli, Eralp, Baylas, Haluk, Atilla, Gül, Lappin, David, Kinane, Denis F.
• Format: Article
• Language: English
• Subjects: Celecoxib; C‐reactive protein; interleukin‐1 beta; omega‐3 fatty acid; osteocalcin; serum
• ISSN: 0022-3492; 1943-3670
• DOI: 10.1902/jop.2006.050164

Background: The aim of this study was to evaluate the effects of selective cyclooxygenase‐2 inhibitor, celecoxib, and omega‐3 fatty acid on serum levels of interleukin 1‐beta (IL‐1β), osteocalcin (OC), and C‐reactive protein (CRP) in experimental periodontitis. Methods: Experimental periodontitis in rats was induced by repeated injection of purified lipopolysaccharide (LPS) derived from Escherichia coli endotoxin. Forty‐seven adult male Sprague‐Dawley rats were divided into five study groups as follows: saline control, LPS, LPS + celecoxib, LPS + omega‐3 fatty acid, and LPS + celecoxib + omega‐3 fatty acid. Celecoxib and omega‐3 fatty acid were given alone or in combination during 14 days of the experimental study period. At the end of the 2‐week protocol, serum samples were obtained, and the rats were sacrificed. Serum samples were analyzed for IL‐1β, OC, and CRP concentrations by enzyme‐linked immunosorbent assay. Defleshed jaws were analyzed morphometrically for alveolar bone loss. Data were evaluated statistically by non‐parametric tests. Results: According to the morphometric measurements, the LPS and drug treatment groups showed significantly higher bone loss than the saline control group (P