Gas Phase Ion Chemistry to Determine Isoaspartate in a Peptide Backbone

Proof of concept evidence is presented for a new method for the determination of isoaspartate, an important post-translational modification. Chemical derivatization is performed using common reagents for the modification of carboxylic acids and shown to yield suitable diagnostic information with reg...

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Bibliographic Details
Published in:Journal of the American Society for Mass Spectrometry 2018-07, Vol.29 (7), p.1339-1344
Main Authors: Ayrton, S. T., Chen, X., Bain, R. M., Pulliam, C. J., Achmatowicz, M., Flick, T. G., Ren, D., Cooks, R. G.
Format: Article
Language:English
Subjects:
Analytical Chemistry
Bioinformatics
Biotechnology
Calibration
Carboxylic acids
Chemistry
Chemistry and Materials Science
Diagnostic systems
Ions
Isoaspartic Acid - analysis
Isomerization
Mass spectrometry
Organic Chemistry
Peptides - analysis
Peptides - chemistry
Protein Processing, Post-Translational
Proteomics
Reagents
Research Article
Spectrometry, Mass, Electrospray Ionization - methods
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Summary:Proof of concept evidence is presented for a new method for the determination of isoaspartate, an important post-translational modification. Chemical derivatization is performed using common reagents for the modification of carboxylic acids and shown to yield suitable diagnostic information with regard to isomerization at the aspartate residue. The diagnostic gas phase chemistry is probed by collision-induced dissociation mass spectrometry, on the timescale of the MS experiment and semi-quantitative calibration of the percentage of isoaspartate in a peptide sample is demonstrated. Graphical Abstract ᅟ
ISSN:1044-0305
1879-1123
DOI:10.1007/s13361-018-1923-0