Gas Phase Ion Chemistry to Determine Isoaspartate in a Peptide Backbone

Proof of concept evidence is presented for a new method for the determination of isoaspartate, an important post-translational modification. Chemical derivatization is performed using common reagents for the modification of carboxylic acids and shown to yield suitable diagnostic information with reg...

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Published in:Journal of the American Society for Mass Spectrometry 2018-07, Vol.29 (7), p.1339-1344
Main Authors: Ayrton, S. T., Chen, X., Bain, R. M., Pulliam, C. J., Achmatowicz, M., Flick, T. G., Ren, D., Cooks, R. G.
Format: Article
Language:English
Subjects:
Analytical Chemistry
Bioinformatics
Biotechnology
Carboxylic acids
Chemistry
Chemistry and Materials Science
Diagnostic systems
Ions
Isomerization
Mass spectrometry
Organic Chemistry
Proteomics
Reagents
Research Article
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cited_by cdi_FETCH-LOGICAL-c372t-15f781c75c75640184597d09a0158321f20a9382969ad26b4228623245334b723
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container_issue 7
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container_title Journal of the American Society for Mass Spectrometry
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creator Ayrton, S. T.
Chen, X.
Bain, R. M.
Pulliam, C. J.
Achmatowicz, M.
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Cooks, R. G.
description Proof of concept evidence is presented for a new method for the determination of isoaspartate, an important post-translational modification. Chemical derivatization is performed using common reagents for the modification of carboxylic acids and shown to yield suitable diagnostic information with regard to isomerization at the aspartate residue. The diagnostic gas phase chemistry is probed by collision-induced dissociation mass spectrometry, on the timescale of the MS experiment and semi-quantitative calibration of the percentage of isoaspartate in a peptide sample is demonstrated. Graphical Abstract ᅟ
doi_str_mv 10.1007/s13361-018-1923-0
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source American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list)
subjects Analytical Chemistry
Bioinformatics
Biotechnology
Carboxylic acids
Chemistry
Chemistry and Materials Science
Diagnostic systems
Ions
Isomerization
Mass spectrometry
Organic Chemistry
Proteomics
Reagents
Research Article
title Gas Phase Ion Chemistry to Determine Isoaspartate in a Peptide Backbone
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