Nodulation and Delayed Nodule Senescence: Strategies of Two Bradyrhizobium Japonicum Isolates with High Capacity to Fix Nitrogen

The purpose of this work was to study further two Bradyrhizobium japonicum strains with high nitrogen-fixing capacity that were identified within a collection of approximately 200 isolates from the soils of Argentina. Nodulation and nitrogen-fixing capacity and the level of expression of regulatory...

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Bibliographic Details
Published in:Current microbiology 2018-08, Vol.75 (8), p.997-1005
Main Authors: López, Silvina M. Y., Sánchez, Ma. Dolores Molina, Pastorino, Graciela N., Franco, Mario E. E., García, Nicolás Toro, Balatti, Pedro A.
Format: Article
Language:English
Subjects:
Argentina
Bacterial Proteins - genetics
Biomass
Biomedical and Life Sciences
Biotechnology
Bradyrhizobium - isolation & purification
Bradyrhizobium - metabolism
Carbon-Carbon Lyases - genetics
Carbon-Carbon Lyases - metabolism
Gene expression
Glycine max - microbiology
Life Sciences
Microbiology
Nitrogen
Nitrogen - metabolism
Nitrogen fixation
Nitrogen Fixation - physiology
Nodulation
Nodules
Plant Root Nodulation - physiology
Plant Roots - microbiology
Root Nodules, Plant - metabolism
Senescence
Symbiosis
Transcription Factors - genetics
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Summary:The purpose of this work was to study further two Bradyrhizobium japonicum strains with high nitrogen-fixing capacity that were identified within a collection of approximately 200 isolates from the soils of Argentina. Nodulation and nitrogen-fixing capacity and the level of expression of regulatory as well as structural genes of nitrogen fixation and the 1-aminocyclopropane-1-carboxylate (ACC) deaminase gene of the isolates were compared with that of E109-inoculated plants. Both isolates of B. japonicum , 163 and 366, were highly efficient to fix nitrogen compared to commercial strain E109. Isolate 366 developed a higher number and larger biomass of nodules and because of this fixed more nitrogen. Isolate 163 developed the same number and nodule biomass than E109. However, nodules developed by isolate 163 had red interiors for a longer period, had a higher leghemoglobin content, and presented high levels of expression of acdS gene, that codes for an ACC deaminase. In conclusion, naturalized rhizobia of the soils of Argentina hold a diverse population that might be the source of highly active nitrogen-fixing rhizobia, a process that appears to be based on different strategies.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-018-1478-0