Genome-wide distribution of histone H3 acetylation in all-trans retinoic acid induced neuronal differentiation of SH-SY5Y cells

With chromatin immunoprecipitation （CHIP） and promoter DNA microarray analyses （ChiP-on-chip）, we analyzed the variations of acetylation on histone H3 in all-trans retinoic acid （RA） induced neuronal cell differentiation. Neuroblastoma SH-SY5Y cells were treated with RA for 24 h and the acetylation...

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Bibliographic Details
Published in:Chinese science bulletin 2009-03, Vol.54 (6), p.1043-1049
Main Authors: Fang, HongBo, Mi, Yang, Wu, NingHua, Zhang, Ye, Shen, YuFei
Format: Article
Language:English
Subjects:
Articles/Molecular Biology
Chemistry/Food Science
DNA芯片
Earth Sciences
Engineering
Humanities and Social Sciences
Life Sciences
multidisciplinary
Physics
Science
Science (multidisciplinary)
乙酰化
全反式维甲酸
全基因
神经元
神经细胞分化
组蛋白H3
诱导分化
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Summary:With chromatin immunoprecipitation （CHIP） and promoter DNA microarray analyses （ChiP-on-chip）, we analyzed the variations of acetylation on histone H3 in all-trans retinoic acid （RA） induced neuronal cell differentiation. Neuroblastoma SH-SY5Y cells were treated with RA for 24 h and the acetylation on histone H3 in the promoter region of the genes was detected. Results showed that, after treatment, the level of acetylation on histone H3 elevated in 597 genes in the genome, and reduced in the other 647 genes compared with those of the control. In summary, we have successfully adopted a high throughput technique to detect and analyze variations of acetylation of histone H3 in human genome at the early phage of RA induced neuronal differentiation of the SH-SY5Y cells.
ISSN:1001-6538
2095-9273
1861-9541
2095-9281
DOI:10.1007/s11434-009-0109-3