Cloning Vectors and Fluorescent Proteins Can Significantly Inhibit Salmonella enterica Virulence in Both Epithelial Cells and Macrophages: Implications for Bacterial Pathogenesis Studies

Plasmid vectors and fluorescent protein reporter systems are commonly used in the study of bacterial pathogenesis. Here we show that they can impair the ability of Salmonella enterica serovar Typhimurium to productively infect either cultured mammalian cells or mice. This has significant implication...

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Bibliographic Details
Published in:Infection and Immunity 2005-10, Vol.73 (10), p.7027-7031
Main Authors: Knodler, Leigh A, Bestor, Aaron, Ma, Caixia, Hansen-Wester, Imke, Hensel, Michael, Vallance, Bruce A, Steele-Mortimer, Olivia
Format: Article
Language:English
Subjects:
Animals
Bacteria
Bacteriology
Biological and medical sciences
Cells, Cultured
Epithelial Cells - microbiology
Fundamental and applied biological sciences. Psychology
Genetic Vectors
Green Fluorescent Proteins - analysis
Green Fluorescent Proteins - genetics
Humans
Macrophages - microbiology
Mice
Microbiology
Miscellaneous
Molecular Pathogenesis
Phagocytosis
Plasmids - genetics
Salmonella enterica
Salmonella Infections - immunology
Salmonella Infections - microbiology
Salmonella typhimurium - growth & development
Salmonella typhimurium - pathogenicity
Virulence - genetics
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Summary:Plasmid vectors and fluorescent protein reporter systems are commonly used in the study of bacterial pathogenesis. Here we show that they can impair the ability of Salmonella enterica serovar Typhimurium to productively infect either cultured mammalian cells or mice. This has significant implications for studies that rely on these systems.
ISSN:0019-9567
1098-5522
DOI:10.1128/IAI.73.10.7027-7031.2005