Reduced mitochondrial DNA content in lymphocytes is associated with insulin resistance and inflammation in patients with impaired fasting glucose

• Published in: Clinical and experimental medicine 2018-08, Vol.18 (3), p.373-382
• Main Authors: Abu Bakar, Mohamad Hafizi, Hairunisa, Nany, Zaman Huri, Hasniza
• Format: Article
• Language: English
• Subjects: Adult; Cholesterol; Cholesterol, HDL - blood; Cholesterol, HDL - immunology; Correlation analysis; Cross-Sectional Studies; DNA, Mitochondrial - blood; DNA, Mitochondrial - immunology; Fasting; Fasting - blood; Female; Glucose Tolerance Test; Glycated Hemoglobin A - immunology; Glycated Hemoglobin A - metabolism; Hematology; High density lipoprotein; Humans; Hyperglycemia; Hyperglycemia - blood; Hyperglycemia - diagnosis; Hyperglycemia - immunology; Inflammation; Insulin; Insulin Resistance; Interleukin 10; Interleukin 6; Interleukin-10 - blood; Interleukin-10 - immunology; Interleukin-6 - blood; Interleukin-6 - immunology; Internal Medicine; Laboratory testing; Lymphocytes; Lymphocytes - chemistry; Lymphocytes - immunology; Male; Medicine; Medicine & Public Health; Metabolism; Microvasculature; Middle Aged; Mitochondria - chemistry; Mitochondrial DNA; Oncology; Original Article; Prediabetic State - blood; Prediabetic State - diagnosis; Prediabetic State - immunology; Risk factors; Statistical analysis; Tumor Necrosis Factor-alpha - blood; Tumor Necrosis Factor-alpha - immunology; Tumor necrosis factor-α
• ISSN: 1591-8890; 1591-9528
• DOI: 10.1007/s10238-018-0495-4

Altered mitochondrial DNA (mtDNA) is the most common denominator to numerous metabolic diseases. The present study sought to investigate the correlation between mtDNA content in lymphocytes and associated clinical risk factors for impaired fasting glucose (IFG). We included 23 healthy control and 42 IFG participants in this cross-sectional study. The measurements of mtDNA content in lymphocytes and pro-inflammatory markers derived from both normal and diseased individuals were quantified. Spearman partial correlation and multivariate statistical analyses were employed to evaluate the association between mtDNA content and other metabolic covariates in IFG. Reduced mtDNA content was observed in the IFG group with microvascular complications than those without complications. The IFG patients with lowest median of mtDNA content had considerably elevated hyperglycemia, insulin resistance and inflammation. The adjusted partial correlation analysis showed that mtDNA content was positively correlated with HDL-cholesterol and IL-10 ( P