The activity of antioxidant defence enzymes in the mussel Mytilus galloprovincialis from the Adriatic Sea

• Published in: Comparative biochemistry and physiology. Toxicology & pharmacology 2005-08, Vol.141 (4), p.366-374
• Main Authors: Borkovic, S S, Saponjic, J S, Pavlovic, S Z, Blagojevic, D P, Milosevic, S M, Kovacevic, T B, Radojicic, R M, Spasic, M B, Zikic, R V, Saicic, Z S
• Format: Article
• Language: English
• Subjects: Adriatic Sea; Animals; Antioxidants - analysis; Catalase; Catalase - metabolism; Environmental Monitoring; Glutathione peroxidase; Glutathione Peroxidase - metabolism; Glutathione reductase; Glutathione Reductase - metabolism; Glutathione Transferase - metabolism; Glutathione- S-transferase; Marine; Mussels; Mytilus - enzymology; Mytilus galloprovincialis; Seasons; Superoxide dismutase; Superoxide Dismutase - metabolism; Water Pollutants, Chemical - toxicity
• ISSN: 1532-0456; 1878-1659
• DOI: 10.1016/j.cbpc.2005.08.001

The activity of the antioxidant defence enzymes superoxide dismutase (SOD, EC 1.15.1.1), catalase (CAT, EC 1.11.1.6), glutathione peroxidase (GSH-Px, EC 1.11.1.9), glutathione reductase (GR, EC 1.6.4.2) and the phase II biotransformation enzyme glutathione-S-transferase (GST, EC 2.5.1.18) in whole mussels ( Mytilus galloprovincialis) were studied. The mussels were collected in winter and in spring at two localities in the Adriatic Sea: Bar Port and Tivat Bay. Our results show that the activities of SOD, GSH-Px and GST were seasonally dependent with higher activities in winter. GR activity was also higher in winter, but only in mussels from Bar Port. In mussels from Tivat Bay, GR activity was lower in winter compared to spring. In addition, a decrease in CAT activity in mussels from Bar Port compared to those from Tivat Bay was found. It can be concluded that seasonal variations should be incorporated into interpretation of biomonitoring studies in mussels.