Production of isodomoic acids A and B as major toxin components of a pennate diatom Nitzschia navis-varingica

In order to obtain more detailed information on the distribution of domoic acid-producing Nitzschia in Asian waters, Nitzschia-like diatoms were isolated primarily from the Philippines and established in culture for analysis by HPLC with fluorescence detection. Out of 58 isolates, 35 Nitzschia-like...

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Bibliographic Details
Published in:Toxicon (Oxford) 2005-12, Vol.46 (8), p.946-953
Main Authors: Kotaki, Yuichi, Furio, Elsa F., Satake, Masayuki, Lundholm, Nina, Katayama, Tsutaki, Koike, Kazuhiko, Fulgueras, Virgilio P., Bajarias, Fe A., Takata, Yoshinobu, Kobayashi, Kenji, Sato, Shigeru, Fukuyo, Yasuwo, Kodama, Masaaki
Format: Article
Language:English
Subjects:
Amnesic shellfish poisoning
Animal poisons toxicology. Antivenoms
Bacillariophyceae
Biological and medical sciences
Brackish
Chromatography, High Pressure Liquid
Diatoms - chemistry
Diatoms - ultrastructure
DNA Primers
Domoic acid
Estuary
Isodomoic acid
Kainic Acid - analogs & derivatives
Kainic Acid - isolation & purification
Marine
Marine Toxins - isolation & purification
Mass Spectrometry
Medical sciences
Microscopy, Electron, Transmission
Nitzschia
Nitzschia navis-varingica
Nuclear Magnetic Resonance, Biomolecular
Pennate diatom
Philippines
Polymerase Chain Reaction
Species Specificity
Spectrophotometry, Ultraviolet
Toxicology
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Summary:In order to obtain more detailed information on the distribution of domoic acid-producing Nitzschia in Asian waters, Nitzschia-like diatoms were isolated primarily from the Philippines and established in culture for analysis by HPLC with fluorescence detection. Out of 58 isolates, 35 Nitzschia-like diatoms from the estuary areas of San Pedro Bay, Tacloban City and Manila Bay showed significant levels of domoic acid production (1.3–5.6 pg/cell). These were identified as Nitzschia navis-varingica. Two isolates from the same locality did not produce domoic acid. Of the 21 isolates from Bulacan Estuary, Manila Bay, none produced domoic acid. They did, however, produce two substances that seemed to be domoic acid derivatives. One of the strains was mass cultured and the substances were extracted, purified and analyzed by LC-MS/MS, proton and 13C NMR, and UV spectra. The produced substances were determined as isodomoic acids A and B. This is the first report of a diatom that produces isodomoic acids A and B as major toxin components.
ISSN:0041-0101
1879-3150
DOI:10.1016/j.toxicon.2005.09.004