Enhanced validamycin production and gene expression at elevated temperature in Streptomyces hygroscopicus subsp, jingangensis 5008

Cultivation shift from 30℃ to 37℃ significantly enhanced validamycin （VAL） production. Analyzed by reverse-transcription PCR, the transcription of three val genes, valA, vaiK and valG, representing the three operons of the cluster was simultaneously increased at elevated temperature. Furthermore, th...

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Bibliographic Details
Published in:Chinese science bulletin 2009-04, Vol.54 (7), p.1204-1209
Main Authors: Li, Lei, Bai, LinQuan, Zhou, XiuFen, Deng, ZiXin
Format: Article
Language:English
Subjects:
Articles/Microbiology
Chemistry/Food Science
Earth Sciences
Engineering
Gene clusters
Humanities and Social Sciences
Life Sciences
multidisciplinary
Operons
Physics
Polymerase chain reaction
Science
Science (multidisciplinary)
Streptomyces hygroscopicus
Temperature effects
Transcription
Validamycins
反转录聚合酶链反应
吸水链霉菌
基因表达
有效霉素
生物合成基因簇
转录基因
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Summary:Cultivation shift from 30℃ to 37℃ significantly enhanced validamycin （VAL） production. Analyzed by reverse-transcription PCR, the transcription of three val genes, valA, vaiK and valG, representing the three operons of the cluster was simultaneously increased at elevated temperature. Furthermore, the transcription of valP and valQ, a pair of two-component regulators in validamycin biosynthetic gene cluster, was also increased at 37℃. Inactivation of valP and valQ reduced validamycin production at 37℃ to the yield level of wild type strain at 30℃, and the valgenes showed reduced expression in the mutant LL-8 at 37℃. These results revealed that the two-component regulator valP and valQ contribute to the elevated validamycin production.
ISSN:1001-6538
2095-9273
1861-9541
2095-9281
DOI:10.1007/s11434-009-0128-0