Chromosomal aberrations in environmentally exposed population in relation to metabolic and DNA repair genes polymorphisms

• Published in: Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis 2007-07, Vol.620 (1-2), p.22-33
• Main Authors: Sram, R J, Beskid, O, Binkova, B, Chvatalova, I, Lnenickova, Z, Milcova, A, Solansky, I, Tulupova, E, Bavorova, H, Ocadlikova, D, Farmer, P B
• Format: Article
• Language: English
• Subjects: Human immunodeficiency virus
• ISSN: 1386-1964; 0027-5107
• DOI: 10.1016/j.mrfmmm.2007.02.019

The capital city of Prague is one of the most polluted localities of the Czech Republic. Therefore, the effect of exposure to carcinogenic polycyclic aromatic hydrocarbons (c-PAHs) adsorbed onto respirable air particles (8h outdoors (N=53). Age- and sex-matched healthy volunteers spending >90% daily time indoors were chosen as controls (N=52). Ambient air particles (PM10, PM2.5) and c-PAHs were monitored using versatile air pollution sampler (VAPS), and personal exposure was evaluated using personal samplers during working shift. Chromosomal aberrations were analyzed by conventional cytogenetic analysis and fluorescent in situ hybridization (FISH). Urinary cotinine plasma levels of vitamins A, E and C, folate, total cholesterol, HDL, LDL cholesterols and triglycerides were also analyzed as possible effect modifiers. Genotypes CYP1A1*2A, CYP1A1*2C, GSTM1, GSTP1, GSTT1, EPHX1, NAT2, hOGG1, XRCC1, XPD, p53 BstI, p53 MspI, MTHFR677, and MS2656 were determined by PCR-based RFLP assays. The following levels of air pollution were recorded during the study period (mean from HiVol sampling): PM10 62.6 mu g/m super(3), c-PAHs 24.7ng /m super(3), B[a]P 3.50ng/m super(3). The conventional cytogenetic analysis did not reveal any differences between the group of policemen exposed to the ambient air pollution and the control group. The cytogenetic analysis by FISH analysis used the whole chromosome painting probes for chromosomes #1 and #4 (Cambio, UK). It detected a significant increase in all studied endpoints in the policemen compared to controls (% AB.C.=0.33+/-0.25 versus 0.24+/-0.18, p