Binding Constant of the Cell-shaped Cavity Formed on a Polymer for Escherichia coli O157

The binding constant of receptors for small molecules, proteins, or antibodies is usually determined based on the concentrations of the ligand, receptor, and their complexes. The binding constant is used as a measure of the affinity between the ligand and the receptor. In the present study, we intro...

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Bibliographic Details
Published in:Analytical Sciences 2018/04/10, Vol.34(4), pp.483-486
Main Authors: SHAN, Xueling, YAMAUCHI, Takuya, SHIIGI, Hiroshi, NAGAOKA, Tsutomu
Format: Article
Language:English
Subjects:
Analytical Chemistry
Antibodies
Binding
Binding constant
cell-shaped cavity
Chemistry
Coordination compounds
E coli
Fluorescence
fluorescent probe
Holes
Ligands
Molecular chains
Molecular imprinting
Polymers
Proteins
Receptors
Substrates
Toxicity
whole cell
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Summary:The binding constant of receptors for small molecules, proteins, or antibodies is usually determined based on the concentrations of the ligand, receptor, and their complexes. The binding constant is used as a measure of the affinity between the ligand and the receptor. In the present study, we introduce a procedure to determine the binding constant of a cell-shaped cavity formed on a polymer by molecular imprinting for a whole cell. To determine the binding constant, we clarified the numbers of cavities and cells, based on the fluorescence of a single cell, and defined their concentrations. We successfully determined the binding constant of the complementary cavity for a whole cell (1.1 × 105 M−1). This is the first report to describe the binding constant of a complementary cavity for a whole cell.
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.17P542