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Change of bacterial communities in sediments along Songhua River in Northeastern China after a nitrobenzene pollution event
More than 100 tons of nitrobenzene and related compounds were released into Songhua River due to the explosion of an aniline production factory in November, 2005. Sediment samples were taken from the heavily polluted drainage canal, one upstream and three downstream river sites. The change of bacter...
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Published in: | FEMS microbiology ecology 2008-09, Vol.65 (3), p.494-503 |
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creator | Li, Dong Yang, Min Li, Zonglai Qi, Rong He, Jizheng Liu, Huijuan |
description | More than 100 tons of nitrobenzene and related compounds were released into Songhua River due to the explosion of an aniline production factory in November, 2005. Sediment samples were taken from the heavily polluted drainage canal, one upstream and three downstream river sites. The change of bacterial community structures along the river was studied by denaturing gradient gel electrophoresis (DGGE) and cloning and sequencing of 16S rRNA genes with five clone libraries constructed and 101 sequences acquired representing 172 clones. Both DGGE profiles and sequences of 16S rRNA genes from clone libraries demonstrated that the contaminated drainage canal and three downstream river sites were similar in that all had Betaproteobacteria, mainly grouped into Comamonadaceae, as the dominant group of bacteria, and all had Firmicutes, primarily as Clostridium spp. These results suggest that these latter two groups of bacteria may play potential roles in degradation and detoxification of nitrobenzene in the present contaminated river environments. |
doi_str_mv | 10.1111/j.1574-6941.2008.00540.x |
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Sediment samples were taken from the heavily polluted drainage canal, one upstream and three downstream river sites. The change of bacterial community structures along the river was studied by denaturing gradient gel electrophoresis (DGGE) and cloning and sequencing of 16S rRNA genes with five clone libraries constructed and 101 sequences acquired representing 172 clones. Both DGGE profiles and sequences of 16S rRNA genes from clone libraries demonstrated that the contaminated drainage canal and three downstream river sites were similar in that all had Betaproteobacteria, mainly grouped into Comamonadaceae, as the dominant group of bacteria, and all had Firmicutes, primarily as Clostridium spp. These results suggest that these latter two groups of bacteria may play potential roles in degradation and detoxification of nitrobenzene in the present contaminated river environments.</description><identifier>ISSN: 0168-6496</identifier><identifier>EISSN: 1574-6941</identifier><identifier>DOI: 10.1111/j.1574-6941.2008.00540.x</identifier><identifier>PMID: 18616580</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Aniline ; Animal and plant ecology ; Animal, plant and microbial ecology ; Bacteria ; Bacteria - genetics ; Bacteria - metabolism ; Biodegradation ; Biodegradation, Environmental ; Biological and medical sciences ; Canals ; China ; clone library ; Cloning ; Comamonadaceae ; Detoxification ; DGGE ; DNA, Bacterial - genetics ; Drainage ; Drainage canals ; Ecology ; Electrophoresis ; Electrophoresis, Gel, Pulsed-Field ; Firmicutes ; Fresh water ecosystems ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Gel electrophoresis ; Gene Library ; Gene sequencing ; Genes ; Genes, Bacterial ; Genes, rRNA ; Geologic Sediments - microbiology ; Microbial ecology ; Microbiology ; Molecular Sequence Data ; Nitrobenzene ; Nitrobenzenes - metabolism ; Phylogeny ; Polymerase Chain Reaction ; Rivers ; Rivers - microbiology ; RNA, Ribosomal, 16S - genetics ; rRNA 16S ; Sediment samplers ; Sediments ; Songhua River ; Synecology ; Various environments (extraatmospheric space, air, water) ; Water Microbiology ; Water Pollutants, Chemical - metabolism ; Water pollution</subject><ispartof>FEMS microbiology ecology, 2008-09, Vol.65 (3), p.494-503</ispartof><rights>2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved 2008</rights><rights>2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5900-88e1b42ce5f8677057cdf850a012256da503ec3457a95950467547c2fa8a566f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20576570$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18616580$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Dong</creatorcontrib><creatorcontrib>Yang, Min</creatorcontrib><creatorcontrib>Li, Zonglai</creatorcontrib><creatorcontrib>Qi, Rong</creatorcontrib><creatorcontrib>He, Jizheng</creatorcontrib><creatorcontrib>Liu, Huijuan</creatorcontrib><title>Change of bacterial communities in sediments along Songhua River in Northeastern China after a nitrobenzene pollution event</title><title>FEMS microbiology ecology</title><addtitle>FEMS Microbiol Ecol</addtitle><description>More than 100 tons of nitrobenzene and related compounds were released into Songhua River due to the explosion of an aniline production factory in November, 2005. Sediment samples were taken from the heavily polluted drainage canal, one upstream and three downstream river sites. The change of bacterial community structures along the river was studied by denaturing gradient gel electrophoresis (DGGE) and cloning and sequencing of 16S rRNA genes with five clone libraries constructed and 101 sequences acquired representing 172 clones. Both DGGE profiles and sequences of 16S rRNA genes from clone libraries demonstrated that the contaminated drainage canal and three downstream river sites were similar in that all had Betaproteobacteria, mainly grouped into Comamonadaceae, as the dominant group of bacteria, and all had Firmicutes, primarily as Clostridium spp. These results suggest that these latter two groups of bacteria may play potential roles in degradation and detoxification of nitrobenzene in the present contaminated river environments.</description><subject>Aniline</subject><subject>Animal and plant ecology</subject><subject>Animal, plant and microbial ecology</subject><subject>Bacteria</subject><subject>Bacteria - genetics</subject><subject>Bacteria - metabolism</subject><subject>Biodegradation</subject><subject>Biodegradation, Environmental</subject><subject>Biological and medical sciences</subject><subject>Canals</subject><subject>China</subject><subject>clone library</subject><subject>Cloning</subject><subject>Comamonadaceae</subject><subject>Detoxification</subject><subject>DGGE</subject><subject>DNA, Bacterial - genetics</subject><subject>Drainage</subject><subject>Drainage canals</subject><subject>Ecology</subject><subject>Electrophoresis</subject><subject>Electrophoresis, Gel, Pulsed-Field</subject><subject>Firmicutes</subject><subject>Fresh water ecosystems</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gel electrophoresis</subject><subject>Gene Library</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Genes, Bacterial</subject><subject>Genes, rRNA</subject><subject>Geologic Sediments - microbiology</subject><subject>Microbial ecology</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Nitrobenzene</subject><subject>Nitrobenzenes - metabolism</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction</subject><subject>Rivers</subject><subject>Rivers - microbiology</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>rRNA 16S</subject><subject>Sediment samplers</subject><subject>Sediments</subject><subject>Songhua River</subject><subject>Synecology</subject><subject>Various environments (extraatmospheric space, air, water)</subject><subject>Water Microbiology</subject><subject>Water Pollutants, Chemical - metabolism</subject><subject>Water pollution</subject><issn>0168-6496</issn><issn>1574-6941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNp1kV9v0zAUxS0EYmXwFcASgreEa8d_EokXVG2ANEBi7Nm6TZ3WVWIXOxkbfHkcWoYEwg_2tfw7R9f3EEIZlCyvV7uSSS0K1QhWcoC6BJACypt7ZHH3cJ8sgKm6UKJRJ-RRSjsAJisBD8kJqxVTsoYF-bHcot9YGjq6wna00WFP2zAMk3ejs4k6T5Ndu8H6MVHsg9_Qy7xtJ6Sf3bWNM_AxxHFrMWW5p8ut80ixyxeKNLvEsLL-u_WW7kPfT6MLntrr7PeYPOiwT_bJ8TwlV-dnX5bviotPb98v31wUrWwAirq2bCV4a2VXK61B6nbd1RIQGOdSrVFCZdtKSI2NbCQIpaXQLe-wRqlUV52SlwfffQxfJ5tGM7jU2r5Hb8OUDAemNVMqg8__Andhij73ZngFSgouKp2pp0dqWg12bfbRDRhvze-hZuDFEcDUYt9F9K1LdxzPP1BSz9zrA_fN9fb2jw-YOWSzM3OWZs7SzCGbXyGbG3N-9iEXWV4d5GHa_0dc_CPOqmcHVYfB4Cbmxq4u8wQqgIazRjTVT1VisbU</recordid><startdate>200809</startdate><enddate>200809</enddate><creator>Li, Dong</creator><creator>Yang, Min</creator><creator>Li, Zonglai</creator><creator>Qi, Rong</creator><creator>He, Jizheng</creator><creator>Liu, Huijuan</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7TV</scope><scope>7UA</scope><scope>F1W</scope><scope>H96</scope><scope>H97</scope><scope>L.G</scope></search><sort><creationdate>200809</creationdate><title>Change of bacterial communities in sediments along Songhua River in Northeastern China after a nitrobenzene pollution event</title><author>Li, Dong ; Yang, Min ; Li, Zonglai ; Qi, Rong ; He, Jizheng ; Liu, Huijuan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5900-88e1b42ce5f8677057cdf850a012256da503ec3457a95950467547c2fa8a566f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Aniline</topic><topic>Animal and plant ecology</topic><topic>Animal, plant and microbial ecology</topic><topic>Bacteria</topic><topic>Bacteria - genetics</topic><topic>Bacteria - metabolism</topic><topic>Biodegradation</topic><topic>Biodegradation, Environmental</topic><topic>Biological and medical sciences</topic><topic>Canals</topic><topic>China</topic><topic>clone library</topic><topic>Cloning</topic><topic>Comamonadaceae</topic><topic>Detoxification</topic><topic>DGGE</topic><topic>DNA, Bacterial - genetics</topic><topic>Drainage</topic><topic>Drainage canals</topic><topic>Ecology</topic><topic>Electrophoresis</topic><topic>Electrophoresis, Gel, Pulsed-Field</topic><topic>Firmicutes</topic><topic>Fresh water ecosystems</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gel electrophoresis</topic><topic>Gene Library</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Genes, Bacterial</topic><topic>Genes, rRNA</topic><topic>Geologic Sediments - microbiology</topic><topic>Microbial ecology</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Nitrobenzene</topic><topic>Nitrobenzenes - metabolism</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction</topic><topic>Rivers</topic><topic>Rivers - microbiology</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>rRNA 16S</topic><topic>Sediment samplers</topic><topic>Sediments</topic><topic>Songhua River</topic><topic>Synecology</topic><topic>Various environments (extraatmospheric space, air, water)</topic><topic>Water Microbiology</topic><topic>Water Pollutants, Chemical - metabolism</topic><topic>Water pollution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, 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sediments along Songhua River in Northeastern China after a nitrobenzene pollution event</atitle><jtitle>FEMS microbiology ecology</jtitle><addtitle>FEMS Microbiol Ecol</addtitle><date>2008-09</date><risdate>2008</risdate><volume>65</volume><issue>3</issue><spage>494</spage><epage>503</epage><pages>494-503</pages><issn>0168-6496</issn><eissn>1574-6941</eissn><abstract>More than 100 tons of nitrobenzene and related compounds were released into Songhua River due to the explosion of an aniline production factory in November, 2005. Sediment samples were taken from the heavily polluted drainage canal, one upstream and three downstream river sites. The change of bacterial community structures along the river was studied by denaturing gradient gel electrophoresis (DGGE) and cloning and sequencing of 16S rRNA genes with five clone libraries constructed and 101 sequences acquired representing 172 clones. Both DGGE profiles and sequences of 16S rRNA genes from clone libraries demonstrated that the contaminated drainage canal and three downstream river sites were similar in that all had Betaproteobacteria, mainly grouped into Comamonadaceae, as the dominant group of bacteria, and all had Firmicutes, primarily as Clostridium spp. These results suggest that these latter two groups of bacteria may play potential roles in degradation and detoxification of nitrobenzene in the present contaminated river environments.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>18616580</pmid><doi>10.1111/j.1574-6941.2008.00540.x</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Aniline Animal and plant ecology Animal, plant and microbial ecology Bacteria Bacteria - genetics Bacteria - metabolism Biodegradation Biodegradation, Environmental Biological and medical sciences Canals China clone library Cloning Comamonadaceae Detoxification DGGE DNA, Bacterial - genetics Drainage Drainage canals Ecology Electrophoresis Electrophoresis, Gel, Pulsed-Field Firmicutes Fresh water ecosystems Freshwater Fundamental and applied biological sciences. Psychology Gel electrophoresis Gene Library Gene sequencing Genes Genes, Bacterial Genes, rRNA Geologic Sediments - microbiology Microbial ecology Microbiology Molecular Sequence Data Nitrobenzene Nitrobenzenes - metabolism Phylogeny Polymerase Chain Reaction Rivers Rivers - microbiology RNA, Ribosomal, 16S - genetics rRNA 16S Sediment samplers Sediments Songhua River Synecology Various environments (extraatmospheric space, air, water) Water Microbiology Water Pollutants, Chemical - metabolism Water pollution |
title | Change of bacterial communities in sediments along Songhua River in Northeastern China after a nitrobenzene pollution event |
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