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Change of bacterial communities in sediments along Songhua River in Northeastern China after a nitrobenzene pollution event

More than 100 tons of nitrobenzene and related compounds were released into Songhua River due to the explosion of an aniline production factory in November, 2005. Sediment samples were taken from the heavily polluted drainage canal, one upstream and three downstream river sites. The change of bacter...

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Published in:FEMS microbiology ecology 2008-09, Vol.65 (3), p.494-503
Main Authors: Li, Dong, Yang, Min, Li, Zonglai, Qi, Rong, He, Jizheng, Liu, Huijuan
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Yang, Min
Li, Zonglai
Qi, Rong
He, Jizheng
Liu, Huijuan
description More than 100 tons of nitrobenzene and related compounds were released into Songhua River due to the explosion of an aniline production factory in November, 2005. Sediment samples were taken from the heavily polluted drainage canal, one upstream and three downstream river sites. The change of bacterial community structures along the river was studied by denaturing gradient gel electrophoresis (DGGE) and cloning and sequencing of 16S rRNA genes with five clone libraries constructed and 101 sequences acquired representing 172 clones. Both DGGE profiles and sequences of 16S rRNA genes from clone libraries demonstrated that the contaminated drainage canal and three downstream river sites were similar in that all had Betaproteobacteria, mainly grouped into Comamonadaceae, as the dominant group of bacteria, and all had Firmicutes, primarily as Clostridium spp. These results suggest that these latter two groups of bacteria may play potential roles in degradation and detoxification of nitrobenzene in the present contaminated river environments.
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Sediment samples were taken from the heavily polluted drainage canal, one upstream and three downstream river sites. The change of bacterial community structures along the river was studied by denaturing gradient gel electrophoresis (DGGE) and cloning and sequencing of 16S rRNA genes with five clone libraries constructed and 101 sequences acquired representing 172 clones. Both DGGE profiles and sequences of 16S rRNA genes from clone libraries demonstrated that the contaminated drainage canal and three downstream river sites were similar in that all had Betaproteobacteria, mainly grouped into Comamonadaceae, as the dominant group of bacteria, and all had Firmicutes, primarily as Clostridium spp. These results suggest that these latter two groups of bacteria may play potential roles in degradation and detoxification of nitrobenzene in the present contaminated river environments.</description><identifier>ISSN: 0168-6496</identifier><identifier>EISSN: 1574-6941</identifier><identifier>DOI: 10.1111/j.1574-6941.2008.00540.x</identifier><identifier>PMID: 18616580</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Aniline ; Animal and plant ecology ; Animal, plant and microbial ecology ; Bacteria ; Bacteria - genetics ; Bacteria - metabolism ; Biodegradation ; Biodegradation, Environmental ; Biological and medical sciences ; Canals ; China ; clone library ; Cloning ; Comamonadaceae ; Detoxification ; DGGE ; DNA, Bacterial - genetics ; Drainage ; Drainage canals ; Ecology ; Electrophoresis ; Electrophoresis, Gel, Pulsed-Field ; Firmicutes ; Fresh water ecosystems ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Gel electrophoresis ; Gene Library ; Gene sequencing ; Genes ; Genes, Bacterial ; Genes, rRNA ; Geologic Sediments - microbiology ; Microbial ecology ; Microbiology ; Molecular Sequence Data ; Nitrobenzene ; Nitrobenzenes - metabolism ; Phylogeny ; Polymerase Chain Reaction ; Rivers ; Rivers - microbiology ; RNA, Ribosomal, 16S - genetics ; rRNA 16S ; Sediment samplers ; Sediments ; Songhua River ; Synecology ; Various environments (extraatmospheric space, air, water) ; Water Microbiology ; Water Pollutants, Chemical - metabolism ; Water pollution</subject><ispartof>FEMS microbiology ecology, 2008-09, Vol.65 (3), p.494-503</ispartof><rights>2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved 2008</rights><rights>2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. 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Sediment samples were taken from the heavily polluted drainage canal, one upstream and three downstream river sites. The change of bacterial community structures along the river was studied by denaturing gradient gel electrophoresis (DGGE) and cloning and sequencing of 16S rRNA genes with five clone libraries constructed and 101 sequences acquired representing 172 clones. 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Sediment samples were taken from the heavily polluted drainage canal, one upstream and three downstream river sites. The change of bacterial community structures along the river was studied by denaturing gradient gel electrophoresis (DGGE) and cloning and sequencing of 16S rRNA genes with five clone libraries constructed and 101 sequences acquired representing 172 clones. Both DGGE profiles and sequences of 16S rRNA genes from clone libraries demonstrated that the contaminated drainage canal and three downstream river sites were similar in that all had Betaproteobacteria, mainly grouped into Comamonadaceae, as the dominant group of bacteria, and all had Firmicutes, primarily as Clostridium spp. These results suggest that these latter two groups of bacteria may play potential roles in degradation and detoxification of nitrobenzene in the present contaminated river environments.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>18616580</pmid><doi>10.1111/j.1574-6941.2008.00540.x</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record>
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subjects Aniline
Animal and plant ecology
Animal, plant and microbial ecology
Bacteria
Bacteria - genetics
Bacteria - metabolism
Biodegradation
Biodegradation, Environmental
Biological and medical sciences
Canals
China
clone library
Cloning
Comamonadaceae
Detoxification
DGGE
DNA, Bacterial - genetics
Drainage
Drainage canals
Ecology
Electrophoresis
Electrophoresis, Gel, Pulsed-Field
Firmicutes
Fresh water ecosystems
Freshwater
Fundamental and applied biological sciences. Psychology
Gel electrophoresis
Gene Library
Gene sequencing
Genes
Genes, Bacterial
Genes, rRNA
Geologic Sediments - microbiology
Microbial ecology
Microbiology
Molecular Sequence Data
Nitrobenzene
Nitrobenzenes - metabolism
Phylogeny
Polymerase Chain Reaction
Rivers
Rivers - microbiology
RNA, Ribosomal, 16S - genetics
rRNA 16S
Sediment samplers
Sediments
Songhua River
Synecology
Various environments (extraatmospheric space, air, water)
Water Microbiology
Water Pollutants, Chemical - metabolism
Water pollution
title Change of bacterial communities in sediments along Songhua River in Northeastern China after a nitrobenzene pollution event
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