Impact of apheresis automation on procedure quality and predictability of CD34+ cell yield

Success of peripheral blood stem cell (PBSC) collections depends on patient biological parameters and stable apheresis device performance. We investigated product quality and factors influencing main apheresis procedure outcomes including CD34+ collection efficiency (CE), product volume or platelet...

Full description

Saved in:
Bibliographic Details
Published in:Journal of clinical apheresis 2018-08, Vol.33 (4), p.494-504
Main Authors: Besson, Nelly, Topholm Bruun, Mie, Stauffer Larsen, Thomas, Nielsen, Christian
Format: Article
Language:English
Subjects:
Antigens, CD34 - analysis
Apheresis
Automation - instrumentation
Blood
Blood Cell Count
Blood Component Removal - instrumentation
Blood Component Removal - methods
Blood Component Removal - standards
CD34+cells
hematopoietic stem cells
Humans
Lymphoma - blood
Multiple Myeloma - blood
Peripheral Blood Stem Cells - cytology
spectra optia
Transplantation, Autologous - methods
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Success of peripheral blood stem cell (PBSC) collections depends on patient biological parameters and stable apheresis device performance. We investigated product quality and factors influencing main apheresis procedure outcomes including CD34+ collection efficiency (CE), product volume or platelet CE. We also assessed different CD34+ cell yield prediction algorithms. Autologous PBSC collections by Spectra Optia from myeloma and lymphoma patients were analyzed. Complete blood count (CBC) from patient preprocedure and from collected products were assessed. (1) Product yield was calculated, (2) Product CBC was correlated with patient preprocedure variables, and (3) Predictions of CD34+ yields based on (a) product CD34+ cell concentration in samples after two or four chamber flushes or (b) traditional CE2 benchmark, were compared. 62 procedures in 41 patients were analyzed. 84% of all procedures were run without operator intervention. Median CD34+ CE2 was 56.9% (48.8%‐65.2%) and quite stable irrespective of patient conditions, with minor influence from patient white blood cell (WBC) precounts (rs = –.47; P 
ISSN:0733-2459
1098-1101
DOI:10.1002/jca.21625