Current perspectives on in vitro maturation and its effects on oocyte genetic and epigenetic profiles

In vitro maturation (IVM), the maturation in culture of immature oocytes, has been used in clinic for more than 20 years. Although IVM has the specific advantages of low cost and minor side effects over controlled ovarian stimulation, the prevalence of IVM is less than 1% of routine in vitro fertili...

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Bibliographic Details
Published in:Science China. Life sciences 2018-06, Vol.61 (6), p.633-643
Main Authors: Lu, Cuiling, Zhang, Yaoyao, Zheng, Xiaoying, Song, Xueling, Yang, Rui, Yan, Jie, Feng, Huailiang, Qiao, Jie
Format: Article
Language:English
Subjects:
Acetylation
Animals
Biomedical and Life Sciences
Cell culture
Culture Media
Cumulus Cells - cytology
Cumulus Cells - physiology
DNA methylation
Embryo transfer
Epigenesis, Genetic
Epigenetics
Fertility
Fertility - genetics
Fertility - physiology
Fertilization in Vitro
Gene expression
Growth factors
Humans
In vitro fertilization
In Vitro Oocyte Maturation Techniques
Life Sciences
Oocytes
Oocytes - cytology
Oocytes - growth & development
Oocytes - physiology
Pituitary (anterior)
Preservation
Review
Time Factors
Transcriptome
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Summary:In vitro maturation (IVM), the maturation in culture of immature oocytes, has been used in clinic for more than 20 years. Although IVM has the specific advantages of low cost and minor side effects over controlled ovarian stimulation, the prevalence of IVM is less than 1% of routine in vitro fertilization and embryo transfer techniques in many reproductive centers. In this review, we searched the MEDLINE database for all full texts and/or abstract articles published in English with content related to oocyte IVM mainly between 2000 and 2016. Many different aspects of the IVM method may influence oocyte potential, including priming, gonadotrophin, growth factors, and culture times. The culture conditions of IVM result in alterations in the oocyte or cumulus cell transcriptome that are not observed under in vivo culture conditions. Additionally, epigenetic modifications, such as DNA methylation or acetylation, are also different between in vitro and in vivo cultured oocytes. In sum, current IVM technique is still not popular and requires more systematic and intensive research to improve its effects and applications. This review will help point our problems, supply evidence or clues for future improving IVM technique, thus assist patients for fertility treatment or preservation as an additional option.
ISSN:1674-7305
1869-1889
DOI:10.1007/s11427-017-9280-4