Comparative Analysis of Flow Cytometry and RQ-PCR for the Detection of Minimal Residual Disease in Philadelphia Chromosome–Positive Acute Lymphoblastic Leukemia after Hematopoietic Stem Cell Transplantation

•Both MRD detection by MFC and RQ-PCR detection of BCR-ABL at the early stage are important predictors of outcome in patients with Ph+-ALL post-transplantation.•MRD detection by MFC and BCR-ABL have complementary roles to predict clinical outcomes for Ph+-ALL at different time points post-transplant...

Full description

Saved in:
Bibliographic Details
Published in:Biology of blood and marrow transplantation 2018-09, Vol.24 (9), p.1936-1943
Main Authors: Zhao, Xiangyu, Zhao, Xiaosu, Chen, Huan, Qin, Yazhen, Xu, Lanping, Zhang, Xiaohui, Liu, Kaiyan, Huang, Xiaojun, Chang, Ying-Jun
Format: Article
Language:English
Subjects:
Adolescent
Adult
BCR-ABL
Child
Child, Preschool
Female
Flow cytometry
Flow Cytometry - methods
Hematopoietic Stem Cell Transplantation - methods
Humans
Male
Middle Aged
MRD
Neoplasm, Residual - diagnosis
Neoplasm, Residual - diagnostic imaging
Philadelphia Chromosome
Precursor Cell Lymphoblastic Leukemia-Lymphoma - diagnosis
Precursor Cell Lymphoblastic Leukemia-Lymphoma - diagnostic imaging
Precursor Cell Lymphoblastic Leukemia-Lymphoma - pathology
Real-Time Polymerase Chain Reaction - methods
Relapse
Transplantation Conditioning - methods
Young Adult
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•Both MRD detection by MFC and RQ-PCR detection of BCR-ABL at the early stage are important predictors of outcome in patients with Ph+-ALL post-transplantation.•MRD detection by MFC and BCR-ABL have complementary roles to predict clinical outcomes for Ph+-ALL at different time points post-transplantation. The aim of this study was to examine the value of minimal residual disease (MRD) detection by multiparameter flow cytometry (MFC) and real-time quantitative PCR (RQ-PCR) at the early stage after hematopoietic stem cell transplantation for predicting relapse and leukemia-free survival (LFS) in Philadelphia chromosome–positive acute lymphoblastic leukemia (Ph+-ALL). Patients who maintained complete molecular remission (BCR-ABL
ISSN:1083-8791
1523-6536
DOI:10.1016/j.bbmt.2018.03.015