Changes in serum anti-Leishmania antibody concentrations measured by time-resolved immunofluorometric assays in dogs with leishmaniosis after treatment

•IgG2 and IgA UFL decrease significantly in dogs with positive respond to treatment.•TR-IFMAs were able to detect decreases of antibodies earlier than the ELISA tested.•The IgG2 and IgA UFL measuring are usefulness for treatment monitoring of CanL The aim of this study was to evaluate the changes in...

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Bibliographic Details
Published in:Veterinary immunology and immunopathology 2018-04, Vol.198, p.65-69
Main Authors: Cantos-Barreda, Ana, Escribano, Damián, Martínez-Subiela, Silvia, Pardo-Marín, Luis, Segarra, Sergi, Cerón, José J.
Format: Article
Language:English
Subjects:
Allopurinol - therapeutic use
Animals
Anti-Leishmania antibodies
Antibodies, Protozoan - blood
Antibodies, Protozoan - immunology
Antiprotozoal Agents - therapeutic use
Canine leishmaniosis
Dog Diseases - drug therapy
Dog Diseases - immunology
Dog Diseases - parasitology
Dogs
Enzyme-Linked Immunosorbent Assay - veterinary
Fluoroimmunoassay - veterinary
Immunoglobulin A - blood
Immunoglobulin A - immunology
Immunoglobulin G - blood
Immunoglobulin G - immunology
Leishmaniasis - blood
Leishmaniasis - drug therapy
Leishmaniasis - immunology
Leishmaniasis - veterinary
Meglumine - therapeutic use
Organometallic Compounds - therapeutic use
Serum
TR-IFMA
Treatment monitoring
Treatment Outcome
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Summary:•IgG2 and IgA UFL decrease significantly in dogs with positive respond to treatment.•TR-IFMAs were able to detect decreases of antibodies earlier than the ELISA tested.•The IgG2 and IgA UFL measuring are usefulness for treatment monitoring of CanL The aim of this study was to evaluate the changes in anti-Leishmania IgG2 and IgA antibodies measured by two time-resolved immunofluorometric assays (TR-IFMAs) recently validated and by means of a commercially available ELISA test in dogs with leishmaniosis after treatment. Serum samples from 16 dogs with clinical leishmaniosis were obtained on days 0, 30 and 180 of treatment. In addition, these serological changes were compared with the clinical signs and selected analytes (total proteins, albumin, globulins and urinary protein:creatinine ratio). Concentrations of IgG2 and IgA by TR-IFMA were significantly lower on days 30 (p 
ISSN:0165-2427
1873-2534
DOI:10.1016/j.vetimm.2018.03.003