Periodontal and endodontic infectious/inflammatory profile in primary periodontal lesions with secondary endodontic involvement after a calcium hydroxide-based intracanal medication

• Published in: Clinical oral investigations 2019-01, Vol.23 (1), p.53-63
• Main Authors: Duque, Thais M., Prado, Maira, Herrera, Daniel R., Gomes, Brenda P. F. A.
• Format: Article
• Language: English
• Subjects: Calcium hydroxide; Collagenase 3; Cytokines; Dental pulp; Dentistry; Endodontics; Enzyme-linked immunosorbent assay; Gelatinase A; Gelatinase B; Gum disease; Inflammation; Interleukin 1; Lipopolysaccharides; Medicine; Neutrophil collagenase; Original Article; Patients; Periodontics; Prostaglandin E2; Root canals; Statistical analysis; Teeth; Tumor necrosis factor-α
• ISSN: 1432-6981; 1436-3771
• DOI: 10.1007/s00784-018-2401-6

Objective The aim of the present study was to investigate the effects of a calcium hydroxide-based intracanal medication (ICM) on periodontal and endodontic infectious/inflammatory contents and on periodontal clinical parameters in teeth with primary periodontal lesion and secondary endodontic involvement. Materials and methods Ten patients with abnormal pulp test results and deep probing depth derived from primary periodontal disease with secondary endodontic involvement were included. Samples were collected from root canals (RC) and periodontal pockets (PP) in order to investigate the microbiological status, levels of endotoxin (LPS), cytokines, and matrix metalloproteinases (MMP), before and after ICM. PCR was used for microbiological assessment. The kinetic-chromogenic LAL assay was used for LPS quantification. Quantikine ELISA kits were used for measurement of IL-1 α, IL-1 β, TNF-α, PGE 2 , MMP-2, MMP-3, MMP-8, MMP-9, and MMP-13 levels. The statistical analyses were made using the Friedman and Wilcoxon tests ( p   0.05); however, the levels of the other MMPs and cytokines were reduced ( p