Method validation of microcystins in water and tissue by enhanced liquid chromatography tandem mass spectrometry

A liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI–MS/MS) method has been developed and validated to identify and quantify trace levels of cyanotoxins or microcystins (MC) in water, bivalves and fish tissue with enhanced sensitivity and specificity. The method enables c...

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Bibliographic Details
Published in:Journal of Chromatography A 2009-04, Vol.1216 (15), p.3147-3155
Main Authors: Mekebri, A., Blondina, G.J., Crane, D.B.
Format: Article
Language:English
Subjects:
Animal, plant and microbial ecology
Applied ecology
Applied sciences
Biological and medical sciences
Ecotoxicology, biological effects of pollution
Exact sciences and technology
Freshwater
Fundamental and applied biological sciences. Psychology
LC–MS/MS
Method validation
Microcystins
MRM
Pollution
Tissue
Water
Water treatment and pollution
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Summary:A liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI–MS/MS) method has been developed and validated to identify and quantify trace levels of cyanotoxins or microcystins (MC) in water, bivalves and fish tissue with enhanced sensitivity and specificity. The method enables confirmation and quantification of six MCs (MC-LA, LF, LR, LW, RR and YR) with a single chromatographic run. The applied chromatography also allows determination of certain MC metabolites (Desmethyl-LR and -RR). By using LC-ESI–MS/MS in multiple reaction monitoring (MRM) mode, the limit of detection and quantitation for the microcystins studied, were determined to be between 0.2 and 1 pg on column (5:1 S/N ratio). These values are below the 2 pg detection limits found in the available literature.
ISSN:0021-9673
DOI:10.1016/j.chroma.2009.01.095