Genotoxicity evaluation of locally produced dental porcelain – An in vitro study using the Ames and Comet assays

• Published in: Toxicology in vitro 2009-09, Vol.23 (6), p.1145-1150
• Main Authors: Noushad, Mohammed, Kannan, Thirumulu Ponnuraj, Husein, Adam, Abdullah, Haswati, Ismail, Abdul Rashid
• Format: Article
• Language: English
• Subjects: Ames assay; Animals; Comet Assay; Dental Porcelain - chemistry; Dental Porcelain - toxicity; DNA damage; DNA Damage - drug effects; Fibroblasts - drug effects; Genotype; Malaysia; Mice; Mutagenicity; Mutagenicity Tests; Porcelain; Salmonella; Salmonella typhimurium - drug effects; Salmonella typhimurium - genetics
• ISSN: 0887-2333; 1879-3177
• DOI: 10.1016/j.tiv.2009.05.025

The aim of this study was to determine the genotoxicity of a locally produced dental porcelain (Universiti Sains Malaysia, Malaysia) using the Ames and Comet assays. In the Ames assay, four genotypic variants of the Salmonella strains (TA98, TA100, TA1537 and TA1535) carrying mutations in several genes were used. The dental porcelain was incubated with these four strains in five different doses both in the presence and absence of metabolic activation (S9) and the result was assessed based on the number of revertant colonies. Concurrently, appropriate positive controls were used so as to validate the test. The average number of revertant colonies per plate treated with locally produced dental porcelain was less than double as compared to that of negative control. In the Comet assay, L929 (CCL-1 ATCC, USA) mouse fibroblast cells were treated with the dental porcelain in three different concentrations along with concurrent negative and positive controls. The tail moment which was used as a measurement of DNA damage was almost equal to that of the negative control, suggesting that the locally produced dental porcelain did not induce any DNA damage. The results indicated that the locally produced dental porcelain is non-genotoxic under the present test conditions.