Comparative pharmacokinetic study of the components of Jia‐Wei‐Kai‐Xin‐San in normal and vascular dementia rats by ultra‐fast liquid chromatography coupled with tandem mass spectrometry

A fast, sensitive, and reliable ultra‐high performance liquid chromatography coupled with tandem mass spectrometry method has been developed and validated for simultaneous quantification of geniposide, polygalaxanthone III, 3,6′‐disinapoyl sucrose, α‐asarone, β‐asarone, poricoic acid A, poricoic aci...

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Bibliographic Details
Published in:Journal of separation science 2018-06, Vol.41 (12), p.2504-2516
Main Authors: Shi, Yiwei, Cao, Cheng, Zhu, Yue, Gao, Ting, Yang, Wen, Liu Mingzhu Qi, Mengqiu, Huang, Renjie, Qian, Dawei, Duan, Jin‐Ao
Format: Article
Language:English
Subjects:
Acids
Analytical chemistry
Chromatography
Dementia
High performance liquid chromatography
Jia‐Wei‐Kai‐Xin‐San
Linearity
Mass spectrometry
Pharmacokinetics
Pharmacology
Rats
Scientific imaging
Spectroscopy
Sucrose
Traditional Chinese medicine
vascular dementia
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Summary:A fast, sensitive, and reliable ultra‐high performance liquid chromatography coupled with tandem mass spectrometry method has been developed and validated for simultaneous quantification of geniposide, polygalaxanthone III, 3,6′‐disinapoyl sucrose, α‐asarone, β‐asarone, poricoic acid A, poricoic acid B, dehydrotumulosic acid, deoxyschizandrin, schizandrin B, and kaempferide in plasma after oral administration of extracts of Jia‐Wei‐Kai‐Xin‐San in normal and vascular dementia rats. The developed method was precise and accurate within the linearity range of the analytes. The lower limits of quantification were 1.04–2.68 ng/mL for all the analytes. Both intra‐ and inter day precision and accuracy of the analytes were all within accepted criteria. The mean extraction recoveries of the analytes and the internal standard from rat plasma were all >60.0%. The validated method had been successfully applied to compare pharmacokinetic profiles of the analytes in plasma of normal and vascular dementia rat treated with herbal extracts. Results indicated that differences existed between normal and vascular dementia model rats except dehydrotumulosic acid and kaempferide, which might be due to the pathology of vascular dementia and pharmacological effect of the analytes. These pharmacokinetic studies might benefit for the mechanism exploration and clinical use of traditional Chinese medicine formulae.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.201701144