Effects of three fungicides alone and in combination on glutathione S-transferase activity (GST) and cytochrome P-450 (CYP 1A1) in the liver and gill of brown trout (Salmo trutta)

• Published in: Marine environmental research 2002-09, Vol.54 (3-5), p.237-240
• Main Authors: Almli, B, Egaas, E, Christiansen, A, Eklo, O.M, Lode, O, Källqvist, T
• Format: Article
• Language: English
• Subjects: Agnatha. Pisces; Animal, plant and microbial ecology; Animals; Applied ecology; Biological and medical sciences; Biomarkers - analysis; Brackish; Cytochrome P-450 CYP1A1 - drug effects; Cytochrome P-450 CYP1A1 - pharmacology; Ecotoxicology, biological effects of pollution; Effects of pollution and side effects of pesticides on vertebrates; Environmental Exposure; fenpropimorph; Freshwater; Fundamental and applied biological sciences. Psychology; Fungicde; Fungicides, Industrial - adverse effects; Fungicides, Industrial - pharmacology; Gills - enzymology; Glutathione transferase; Glutathione Transferase - drug effects; Glutathione Transferase - pharmacology; Liver - enzymology; Marine; Salmo trutta; Trout; Trout - physiology; Water Pollutants, Chemical - adverse effects; Water Pollutants, Chemical - pharmacology
• ISSN: 0141-1136; 1879-0291
• DOI: 10.1016/S0141-1136(02)00157-5

In order to evaluate the gill glutathione S-transferase (GST) activity as a biomarker of effect of fungicide exposure in juvenile brown trout (Salmo trutta), the fungicides propiconazole {(R,S)-1-[2-(2,4-diclophenyl)-4-propyl-1,3-dioolan-2-ylmetyl]-1H-1,2,4-triazole} and fenpropimorph {(±)-cis-4-[3-(4-tert-butylphenyl)-2-metyl propyl]-2,6 dimetylmorfolinc} were administrated in the water separately and together in a static system (80 μg/l for each pesticide) for 5 days. The combined fungicides gave a significant decrease in gill GST activity towards 1-chloro-2,4-dinitrobenzene (CDNB), whilst hepatic GST-activity was not significantly changed. Furthermore, continuous exposure to 540 ug/l thiabendazole{ 2-(thiazol-4′-yl)benzimidazole} in a flow-through system for 4 days significantly increased the gill glutathione S-transferase (GST) activity towards CDNB, whilst hepatic GST and cytochrome P450 (CYP 1A) activities were not increased by the treatment.