Molecular cloning and characterization of a brassinosteriod biosynthesis-related gene PtoDWF4 from Populus tomentosa

Brassinosteroids (BRs) as steroid hormones play an important role in plant growth and development. However, little is known about how BRs affect secondary wall biosynthesis in woody plants. In this study, we cloned and characterized PtoDWF4, a homologus gene of Arabidopsis DWF4 encoding a cytochrome...

Full description

Saved in:
Bibliographic Details
Published in:Tree physiology 2018-09, Vol.38 (9), p.1424-1436
Main Authors: Shen, Yun, Li, Yongli, Xu, Dan, Yang, Chen, Li, Chaofeng, Luo, Keming
Format: Article
Language:English
Subjects:
Arabidopsis Proteins - genetics
Brassinosteroids - biosynthesis
Cell Wall - genetics
Cell Wall - metabolism
Cloning, Molecular
Cytochrome P-450 Enzyme System - genetics
Gene Expression Regulation, Plant
Mutation
Phylogeny
Plant Cells - metabolism
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Stems - genetics
Plant Stems - metabolism
Plants, Genetically Modified
Populus - genetics
Populus - growth & development
Populus - metabolism
Wood - genetics
Wood - growth & development
Xylem - genetics
Xylem - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Brassinosteroids (BRs) as steroid hormones play an important role in plant growth and development. However, little is known about how BRs affect secondary wall biosynthesis in woody plants. In this study, we cloned and characterized PtoDWF4, a homologus gene of Arabidopsis DWF4 encoding a cytochrome P450 protein, from Populus tomentosa. qRT-PCR analysis showed that PtoDWF4 was highly expressed in stems, especially in xylem. Overexpression of PtoDWF4 (PtoDWF4-OE) in poplar promoted growth rate and biomass yield, increased area and cell layers of xylem. Transgenic plants showed a significant increase in plant height and stem diameter compared with the wild type. In contrast, the CRISPR/Cas9-generated mutation of PtoDWF4 (PtoDWF4-KO) resulted in significantly decreased biomass production in transgenic plants. Further studies revealed that constitutive expression of PtoDWF4 up-regulated the expression of secondary cell wall (SCW) biosynthesis-related genes, whereas knock-out of PtoDWF4 down-regulated their expression. Quantitative analysis of cell wall components showed a significant increase in PtoDWF4-OE lines but a reduction in PtoDWF4-KO lines compared with wild-type plants. Taken together, our results indicate that PtoDWF4 plays a positive role in improving growth rate and elevating biomass production in poplar.
ISSN:1758-4469
1758-4469
DOI:10.1093/treephys/tpy027