A cytochrome P450 monooxygenase cDNA (CYP71A10) confers resistance to linuron in transgenic Nicotiana Tabacum

The isolation of a Glycine max cytochrome P450 monooxygenase (P450) cDNA designated CYP71A10 that conferred linuron resistance to laboratory-grown, transgenic Nicotiana tabacum seedlings was previously reported. A nonsegregating transgenic N. tabacum line has been established that possesses two inde...

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Bibliographic Details
Published in:Weed science 2000-05, Vol.48 (3), p.291-295
Main Authors: Siminszky, B, Sheldon, B S, Corbin, F T, Dewey, R E
Format: Article
Language:English
Subjects:
Glycine max
Nicotiana tabacum
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Summary:The isolation of a Glycine max cytochrome P450 monooxygenase (P450) cDNA designated CYP71A10 that conferred linuron resistance to laboratory-grown, transgenic Nicotiana tabacum seedlings was previously reported. A nonsegregating transgenic N. tabacum line has been established that possesses two independent copies of the G. max CYP71A10 transgene. Five-week-old progeny plants of this selected line were grown in a controlled environmental chamber and treated with linuron using either pretransplant incorporated (PTI) or postemergence (POST) applications. CYP71A10-transformed N. tabacum was more tolerant to linuron than the wild type for both application methods. The transgenic N. tabacum line tolerated an approximately 16-fold and 12-fold higher rate of linuron than wild-type N. tabacum when the herbicide was applied PTI or POST, respectively. These results provide evidence that plant-derived P450 genes can be employed effectively to confer herbicide resistance to transgenic plants. Nomenclature: Cytochrome P450; linuron; Glycine max L. Merr. aDare', soybean; Nicotiana tabacum L. aSR1', tobacco.
ISSN:0043-1745
DOI:10.1043/0043-1745(2000)048<0291:ACPMCC>2.0.CO;2