Glucose oxidase assisted visual detection of glucose using oxygen deficient α-MoO3-x nanoflakes

An optical method is described for the quantitation of glucose by using oxygen-deficient α-MoO 3-x nanoflakes. It is based on the use of glucose oxidase (GOx) which produces hydrogen peroxide on oxidation of glucose. Hydrogen peroxide then oxidizes the α-MoO 3-x nanoflakes, and this results in a vis...

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Bibliographic Details
Published in:Mikrochimica acta (1966) 2018-01, Vol.185 (1), p.1-8, Article 65
Main Authors: Nerthigan, Yowan, Sharma, Amit Kumar, Pandey, Sunil, Sharma, Krishna Hari, Shahnawaz Khan, M., Hang, Da-Ren, Wu, Hui-Fen
Format: Article
Language:English
Subjects:
Analytical Chemistry
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Color
Glucose
Glucose oxidase
Hydrogen peroxide
Microengineering
Molybdenum trioxide
Nanochemistry
Nanotechnology
Original Paper
Oxidation
Photometry
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Summary:An optical method is described for the quantitation of glucose by using oxygen-deficient α-MoO 3-x nanoflakes. It is based on the use of glucose oxidase (GOx) which produces hydrogen peroxide on oxidation of glucose. Hydrogen peroxide then oxidizes the α-MoO 3-x nanoflakes, and this results in a visible color change from blue to colorless. The color change can be measured photometrically at 740 nm. The method has a 68 nM detection limit. Graphical Abstract Mechanism of glucose detection using blue colored oxygen deficient 2D α-MoO 3-x nanoflakes. Hydrogen peroxide (H 2 O 2 ) is formed as a by-product in the conversion of glucose to glucono-1,5-lactone by glucose oxidase (GO x ). In the presence of H 2 O 2 , the oxygen vacancies in α-MoO 3-x nanoflakes are filled up, and this leads to the loss of blue color of the nanoflakes because they are converted back to colorless bulk α-MoO 3 .
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-017-2612-6