S,N-doped carbon dots as a fluorescent probe for bilirubin

Carbon dots doped with sulfur and nitrogen (S,N-CDs) were utilised to design a paper-stripe based fluorescent probe for the detection of bilirubin. The S,N-CDs were synthesized through a microwave assisted route by using citric acid as carbon source and L-cysteine as a source of nitrogen and sulfur....

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Bibliographic Details
Published in:Mikrochimica acta (1966) 2018-01, Vol.185 (1), p.11-11, Article 11
Main Authors: Anjana, R. R., Anjali Devi, J. S., Jayasree, M., Aparna, R. S., Aswathy, B., Praveen, G. L., Lekha, G. M., Sony, George
Format: Article
Language:English
Subjects:
Analytical Chemistry
Bilirubin
Carbon
Carbon dots
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Citric acid
Cysteine
Ethylenediaminetetraacetic acid
Fluorescence
Investigations
Microengineering
Nanochemistry
Nanotechnology
Nitrogen
Original Paper
Quenching
Selectivity
Sulfur
Urine
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Summary:Carbon dots doped with sulfur and nitrogen (S,N-CDs) were utilised to design a paper-stripe based fluorescent probe for the detection of bilirubin. The S,N-CDs were synthesized through a microwave assisted route by using citric acid as carbon source and L-cysteine as a source of nitrogen and sulfur. The S,N-CDs exhibit bright blue fluorescence emission with a peak at 452 nm. Fluorescence is quenched by Fe(III) but selectively restored by bilirubin. The quenched fluorescent probe exhibit significant selectivity and sensitivity for bilirubin in the 0.2 nM to 2 nM concentration range, with a 0.12 nM detection limit. The method was applied to the determination of bilirubin in spiked human serum and urine samples. The method was used to design a paper based test stripe as a point of care device for visual bilirubin detection. Graphical abstract Schematic representation of sulphur and nitrogen doped carbon dots whose fluorescence is quenched by Fe(III) and turned on by bilirubin. Photograph of the corresponding system under day light and UV shows the feasibility of the phenomenon. The applicability of the assay was further extended by impregnating the probe on a filter paper.
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-017-2574-8