The effect of interaction between EtOH dosage and exposure time on gene expression in DPSC

Alcohol (EtOH) dosage and exposure time can affect gene expression. However, whether there exists synergistic effect is unknown. Here, we analyzed the hDPSC gene microarray dataset GSE57255 downloaded from Gene Expression Omnibus and found that the interaction between EtOH dosage and exposure time o...

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Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 2019-05, Vol.111 (3), p.500-507
Main Authors: Li, Jianqiang, Wang, Zhirui, Qiu, Weiliang, Yang, Ji-Jiang, Wang, Qing, Chen, Shi, Pan, Hui
Format: Article
Language:English
Subjects:
Alcohol consumption
Alcohol Drinking
Dental Pulp - metabolism
DPSC
Ethanol - pharmacology
FDR
Gene Expression Profiling
Humans
KEGG
Microarray Analysis
Stem Cells - metabolism
Synergistic effect
Time
Two-way factorial design
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Summary:Alcohol (EtOH) dosage and exposure time can affect gene expression. However, whether there exists synergistic effect is unknown. Here, we analyzed the hDPSC gene microarray dataset GSE57255 downloaded from Gene Expression Omnibus and found that the interaction between EtOH dosage and exposure time on gene expression are statistically significant for two probes: 201917_s_at near gene SLC25A36 and 217649_at near gene ZFAND5. GeneMania showed that SLC25A36 and ZFAND5 were related to 20 genes, three of which had alcohol-related functions. WebGestalt revealed that the 22 genes were enriched in 10 KEGG pathways, four of which are related to alcoholic diseases. We explored the possible nonlinear interaction effect and got 172 gene probes with significant p-values. However, no significantly enriched pathways based on the 172 probes were detected. Our analyses indicated a possible molecular mechanism that could help explain why alcohol consumption has both deleterious and beneficial effects on human health. •We investigated the synergistic effect of EtOH dosage and exposure time on gene expression.•Expression of 201917_s_at (SLC25A36) and 217649_at (ZFAND5) affected by interaction between EtOH dosage and exposure time.•SLC25A36 and ZFAND5 have not been linked with alcohol consumption yet.•SLC25A36 and ZFAND5 are related to 3 alcohol-related genes via GeneMania analysis.•The 22 genes obtained via GeneMania analysis enriched in 4 alcohol-related KEGG pathways.
ISSN:0888-7543
1089-8646
DOI:10.1016/j.ygeno.2018.03.009