Lymphoblast-derived integration-free iPSC line AD-TREM2-1 from a 67year-old Alzheimer's disease patient expressing the TREM2 p.R47H variant

Human lymphoblast cells from a male diagnosed with Alzheimer's disease (AD) expressing the TREM2 p.R47H variant were used to generate integration-free induced pluripotent stem cells (iPSCs) by over-expressing episomal-based plasmids harbouring OCT4, SOX2, NANOG, LIN28, c-MYC and L-MYC. AD-TREM2...

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Bibliographic Details
Published in:Stem cell research 2018-05, Vol.29, p.60-63
Main Authors: Martins, Soraia, Yigit, Hatice, Bohndorf, Martina, Graffmann, Nina, Fiszl, Aurelian Robert, Wruck, Wasco, Sleegers, Kristel, Van Broeckhoven, Christine, Adjaye, James
Format: Article
Language:English
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Summary:Human lymphoblast cells from a male diagnosed with Alzheimer's disease (AD) expressing the TREM2 p.R47H variant were used to generate integration-free induced pluripotent stem cells (iPSCs) by over-expressing episomal-based plasmids harbouring OCT4, SOX2, NANOG, LIN28, c-MYC and L-MYC. AD-TREM2–1 was defined as pluripotent based on (i) expression of pluripotency-associated markers (ii) embryoid body-based differentiation into cell types representative of the three germ layers and (iii) the similarity between the transcriptome of the iPSC line and the human embryonic stem cell line H1 with a Pearson correlation of 0.947.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2018.03.011