High sensitive and multiple detection of acute myocardial infarction biomarkers based on a dual-readout immunochromatography test strip

• Published in: Nanomedicine 2018-06, Vol.14 (4), p.1257-1266
• Main Authors: Gong, Xiaoqun, Zhang, Bo, Piao, Jiafang, Zhao, Qian, Gao, Weichen, Peng, Weipan, Kang, Qi, Zhou, Dianming, Shu, Guiming, Chang, Jin
• Format: Article
• Language: English
• Subjects: Acute myocardial infarction (AMI); Animals; Biomarkers - metabolism; Chromatography, Affinity - methods; Dual-readout; Electron Spin Resonance Spectroscopy; Humans; Immunochromatography test strip (ICTS); Kinetics; Lactoferrin - metabolism; Magnetic nanoparticles (MNPs); Male; Myocardial Infarction - diagnosis; Myocardial Infarction - metabolism; Oxidation-Reduction; Rats; Ubiquinone - analogs & derivatives; Ubiquinone - metabolism
• ISSN: 1549-9634; 1549-9642
• DOI: 10.1016/j.nano.2018.02.013

Immunochromatography test strip (ICTS) displayed high advantages in screening acute myocardial infarction (AMI) biomarkers. However, the low sensitivity and nonquantitative results seriously limited its clinical application. Herein, we designed a highly sensitive, quantitative and dual-readout ICTS for assaying multiple AMI biomarkers based on magnetic nanoparticles (MNPs) quenching the fluorescence of Cy5, which was labeled on capture antibodies on test (T) lines. The changes of fluorescent intensity caused by MNPs nanoprobes enabled us to sensitively quantify cTnI and CK-MB for early diagnosis of AMI in 15 min with a corresponding detection limit of 0.049 ng/mL and 0.085 ng/mL, respectively. Meanwhile, the aggregations of MNPs on T lines allowed colorimetric readout in 2 min for rapid diagnosis of emergent and severe AMI patients. Furthermore, the detection results of 30 clinical serum samples were coincident with those by electrochemiluminescence immunoassay. So this approach is promising a new avenue for clinical diagnosis and prognosis of AMI. In this work, we designed a highly sensitive, multiplex-detection and dual-readout ICTS for the assay of AMI biomarkers based on magnetic probes quenching the fluorescence of Cy5 dyes immobilized on the test (T) line. Aggregation of magnetic probes on T line generated colorimetric readout for quick qualitative detection of emergent and severe patients; fluorescent signal by magnetic probes largely quenching Cy5 on T lines achieved highly sensitive and quantitative detection for early diagnosis. (A): The scheme of ICTS platform for simultaneous qualitative and quantitative detection of cTnI and CK-MB. (B): The application of the dual-readout ICTS for assaying the biomarkers (a black colored T line appeared in bright-field and a fluorescence attenuated T line in dark-field). [Display omitted]