Peptide‐Based Scaffold for Nitric Oxide Induced Differentiation of Neuroblastoma Cells

Nitric oxide is a gaseous messenger involved in neuronal differentiation, development and synaptogenesis, in addition to many other physiological functions. Therefore, it is imperative to maintain an optimal nitric oxide concentration to ensure its biochemical function. A sustained nitric oxide rele...

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Bibliographic Details
Published in:Chembiochem : a European journal of chemical biology 2018-06, Vol.19 (11), p.1127-1131
Main Authors: Pal, Hilal Ahmad, Singh, Anamika, Sheikh, Parvaiz A., Panjla, Apurva, Kumar, Ashok, Verma, Sandeep
Format: Article
Language:English
Subjects:
Axonogenesis
Cell differentiation
Differentiation (biology)
immunoassays
Morphometry
Neuroblastoma
Neuroblasts
neurochemistry
Nitric oxide
nitrogen oxides
peptides
Proteins
Scaffolds
Synaptogenesis
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Summary:Nitric oxide is a gaseous messenger involved in neuronal differentiation, development and synaptogenesis, in addition to many other physiological functions. Therefore, it is imperative to maintain an optimal nitric oxide concentration to ensure its biochemical function. A sustained nitric oxide releasing scaffold, which supports neuronal cell differentiation, as determined by morphometric analysis of neurite outgrowth, is described. Moreover, the effect of nitric oxide on the neuroblastoma cell line was also confirmed by immunofluorescent analysis of neuronal nuclear protein (NeuN), specific neuronal marker and neurofilament (NF) protein, which revealed a significant increase in their expression levels, in comparison with undifferentiated cells. Tracking the messenger: A phenylalanine‐based scaffold functionalised with a nitric oxide donor moiety has been designed and explored for the differentiation of the Neuro2a cell line. The effects of nitric oxide are confirmed by immunohistochemistry of specific neuronal marker proteins.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.201800065