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Reduction of Membrane Protein CRIM1 Decreases E-Cadherin and Increases Claudin-1 and MMPs, Enhancing the Migration and Invasion of Renal Carcinoma Cells

CRIM1 is a membrane protein that has been reported to be related to cell proliferation. CRIM1 is expressed in renal carcinoma cells, but its involvement in proliferation and malignant transformation remains unclear. We analyzed whether alterations in the characteristics of cancer cells are observed...

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Published in:Biological & pharmaceutical bulletin 2018/04/01, Vol.41(4), pp.604-611
Main Authors: Ogasawara, Nobutaka, Kudo, Tamami, Sato, Masaki, Kawasaki, Yasushi, Yonezawa, Sei, Takahashi, Satoru, Miyagi, Yohei, Natori, Yasuhiro, Sugiyama, Akinori
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creator Ogasawara, Nobutaka
Kudo, Tamami
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Miyagi, Yohei
Natori, Yasuhiro
Sugiyama, Akinori
description CRIM1 is a membrane protein that has been reported to be related to cell proliferation. CRIM1 is expressed in renal carcinoma cells, but its involvement in proliferation and malignant transformation remains unclear. We analyzed whether alterations in the characteristics of cancer cells are observed following knockdown of CRIM1. Decreased expression of CRIM1 did not affect proliferation or anchorage-independent growth. The results of wound healing and invasion assays showed that reduced expression of CRIM1 increased cells’ migratory and invasive abilities. Expression analysis of factors involved in migration and invasion in CRIM1-knockdown cells revealed that expression of the cell adhesion factor E-cadherin declined and expression of claudin-1, which is upregulated in metastatic cancer cells, increased. In addition, increased expression of matrix metalloproteinase (MMP) 2 and MMP9, protease essential for cancer cell invasiveness, was observed. Furthermore, an increase in phosphorylated focal adhesion kinase (FAK), which increases cell migration, was observed. Increased expression of the E-cadherin transcription repressors Snail, Slug, and ZEB-1 were observed, and mRNA levels of E-cadherin were decreased. Therefore, expression of E-cadherin is thought to be decreased by both suppression of E-cadherin mRNA expression and promotion of degradation of the E-cadherin protein. In addition, expression of CRIM1 was decreased in renal cancer cells undergoing epithelial–mesenchymal transition (EMT) stimulated by tumor necrosis factor alpha (TNF-α). Thus, CRIM1 regulates the expression of several EMT-related factors and appears to play a role in suppressing migration and invasion through control of EMT.
doi_str_mv 10.1248/bpb.b17-00990
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source Free Full-Text Journals in Chemistry
subjects Adhesion
Cancer
Cell adhesion
Cell adhesion & migration
Cell migration
Cell proliferation
claudin-1
CRIM1
E-cadherin
epithelial–mesenchymal transition
Focal adhesion kinase
Gelatinase B
Gene expression
Invasiveness
Kidney cancer
Kinases
Matrix metalloproteinase
Membrane proteins
Mesenchyme
Metalloproteinase
Metastases
Proteins
Renal cell carcinoma
Repressors
Snail protein
Transcription factors
tumor necrosis factor alpha
Tumor necrosis factor-TNF
Tumor necrosis factor-α
Wound healing
title Reduction of Membrane Protein CRIM1 Decreases E-Cadherin and Increases Claudin-1 and MMPs, Enhancing the Migration and Invasion of Renal Carcinoma Cells
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