The water-soluble non-starch polysaccharides from bananas display immunomodulatory properties on cultured macrophages

• Published in: Food research international 2016-09, Vol.87, p.125-133
• Main Authors: Sansone, Marcelo, Sansone, Ana Cláudia Miranda Brito, Shiga, Tania Misuzu, Nascimento, João Roberto Oliveira do
• Format: Article
• Language: English
• Subjects: Banana; Biological response modifiers; Non-starch polysaccharides
• ISSN: 0963-9969; 1873-7145
• DOI: 10.1016/j.foodres.2016.07.003

Some diet components, such as certain indigestible polysaccharides from edible plants, may interact with the gut-associated lymphoid tissue and improve the host immune response to pathogens. The non-starch polysaccharides (NSP) from bananas are non-digestible carbohydrates that resemble some immunomodulatory polysaccharides occurring in the cell wall of cereals. Based on this similarity, the effects of the water-soluble NSP from two banana cultivars (Nanicão and Thap Maeo) on the phagocytic activity, nitric oxide (NO) and cytokines produced by cultured macrophages were investigated. An investigation into the monosaccharide composition and the oligomers released by enzymatic hydrolysis of the ultra-filtered fraction above 50kDa and the heat-treated fraction of water-soluble NSP from both cultivars revealed they are mostly composed of mannan and galacturonans (homogalacturonan, xylogalacturonan and rhamnogalacturonan). The NSP tested were able to activate the macrophages, but the effects on the phagocytic activity and the release of tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6) and nitric oxide production were dependent on the polysaccharide concentration and the cultivar origin. Despite some specific differences, the NSP from Nanicão and Thap Maeo banana fruits may be considered prospective food immunomodulators, which contribute to the promotion of a more responsive immune system. [Display omitted] •Banana non-starch polysaccharides modulated RAW 264.7 macrophages•Purification of polysaccharides affected macrophage activation and phagocytosis•Some effects of polysaccharides were related to the banana cultivar of origin•Banana immunomodulatory polysaccharides were mainly mannans and galacturonans