Confirmation of an Enzyme-Linked Immunosorbent Assay to Detect Fluometuron in Soil

• Published in: Weed technology 2001-10, Vol.15 (4), p.669-675
• Main Authors: Mark W. Shankle, Shaw, David R., Michele Boyette
• Format: Article
• Language: English
• Subjects: Acid soils; Agricultural soils; Agronomy. Soil science and plant productions; Biological and medical sciences; Chemical control; Enzyme linked immunosorbent assay; Filter strips; Forest soils; Fundamental and applied biological sciences. Psychology; Herbicides; Parasitic plants. Weeds; Phytopathology. Animal pests. Plant and forest protection; Riparian areas; Riparian soils; Soil and water pollution; Soil samples; Soil science; Soil water; Weeds
• ISSN: 0890-037X; 1550-2740
• DOI: 10.1614/0890-037X(2001)015[0669:COAELI]2.0.CO;2

Research was conducted to compare the results of an enzyme-linked immunosorbent assay (ELISA) to high-performance liquid chromatography (HPLC) for detecting fluometuron in the environment. A linear relationship for HPLC $(R^{2}>0.90)$ and ELISA $(R^{2}>0.66)$ analysis was observed between the natural logarithm of the detected fluometuron concentrations regressed against time in soil collected from a cropped area, a grass filter strip, and a riparian forest. Both methods detected the same initial fluometuron concentration (y-intercept) for two of the three soils evaluated. The ELISA and HPLC measurements of fluometuron concentrations compared favorably with r values from 0.83 to 0.98. Predicted fluometuron half-lives determined from HPLC and ELISA measurements were: 110 and 112 d in the cropped watershed, 28 and 29 d in the riparian area, and 11 and 11 d in the grass filter strip, respectively. Results from both techniques indicated shorter half-lives in soil from the grass filter strip and riparian area than in cropped area soil. There was an inverse correlation between predicted half-lives and soil organic matter, pH, clay, and cation exchange capacity.